Neospora caninum and bone marrow-derived dendritic cells: parasite survival, proliferation, and induction of cytokine expression

Strohbusch, M.; Muller, N.; Hemphill, A.; Margos, M.; Grandgirard, D.; Leib, Stephen; Greif, G.; Gottstein, B. (2009). Neospora caninum and bone marrow-derived dendritic cells: parasite survival, proliferation, and induction of cytokine expression. Parasite immunology, 31(7), pp. 366-372. Oxford: Blackwell Scientific Publications 10.1111/j.1365-3024.2009.01112.x

[img] Text
__nas-ifik.unibe.ch_ifik_Forschung_Gr_Leib_SHARED GROUP FOLDER_own papers_STROHBUSCH_et_al-2009-Parasite_Immunology.pdf - Published Version
Restricted to registered users only
Available under License Publisher holds Copyright.

Download (398kB) | Request a copy

Dendritic cells (DCs) represent the first line defence of the innate immune system following infection with pathogens. We exploratively addressed invasion and survival ability of Neospora caninum, a parasite causing abortion in cattle, in mouse bone marrow DCs (BMDCs), and respective cytokine expression patterns. Immature BMDCs were exposed to viable (untreated) and nonviable parasites that had been inactivated by different means. Invasion and/or internalization, as well as intracellular survival and proliferation of tachyzoites were determined by NcGRA2-RT-PCR and transmission electron microscopy (TEM). Cytokine expression was evaluated by reverse transcription (RT)-PCR and cytokine ELISA. Transmission electron microscopy of DCs stimulated with untreated viable parasites revealed that N. caninum was able to invade and proliferate within BMDCs. This was confirmed by NcGRA2-RT-PCR. On the other hand, no viable parasite organisms were revealed by TEM when exposing BMDCs to inactivated parasites (nonviability demonstrated by NcGRA2-RT-PCR). Cytokine expression analysis (as assessed by both RT-PCR and ELISA) demonstrated that both viable and nonviable parasites stimulated mBMDCs to express IL-12p40, IL-10 and TNF-alpha, whereas IL-4 RNA expression was not detected. Thus, exposure of mBMDCs to both viable and nonviable parasites results in the expression of cytokines that are relevant for a mixed Th1/Th2 immune response.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Service Sector > Institute for Infectious Diseases
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Parasitology

UniBE Contributor:

Müller, Norbert; Hemphill, Andrew; Leib, Stephen and Gottstein, Bruno

Subjects:

500 Science > 570 Life sciences; biology
600 Technology > 610 Medicine & health

ISSN:

0141-9838

Publisher:

Blackwell Scientific Publications

Language:

English

Submitter:

Factscience Import

Date Deposited:

04 Oct 2013 15:25

Last Modified:

19 Jul 2018 16:06

Publisher DOI:

10.1111/j.1365-3024.2009.01112.x

PubMed ID:

19527452

Web of Science ID:

000266678800003

BORIS DOI:

10.7892/boris.38346

URI:

https://boris.unibe.ch/id/eprint/38346 (FactScience: 221181)

Actions (login required)

Edit item Edit item
Provide Feedback