Is ITS-2 rDNA suitable marker for genetic characterization of Sarcoptes mites from different wild animals in different geographic areas?

Alasaad, S.; Soglia, D.; Spalenza, V.; Maione, S.; Soriguer, R.C.; Perez, J.M.; Rasero, R.; Degiorgis, Marie Pierre; Nimmervoll, H.; Zhu, X.Q.; Rossi, L. (2009). Is ITS-2 rDNA suitable marker for genetic characterization of Sarcoptes mites from different wild animals in different geographic areas? Veterinary parasitology, 159(2), pp. 181-5. Amsterdam: Elsevier 10.1016/j.vetpar.2008.10.001

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The present study examined the relationship among individual Sarcoptes scabiei mites from 13 wild mammalian populations belonging to nine species in four European countries using the second internal transcribed spacer (ITS-2) of nuclear ribosomal DNA (rDNA) as genetic marker. The ITS-2 plus primer flanking 5.8S and 28S rDNA (ITS-2+) was amplified from individual mites by polymerase chain reaction (PCR) and the amplicons were sequenced directly. A total of 148 ITS-2+ sequences of 404bp in length were obtained and 67 variable sites were identified (16.59%). UPGMA analyses did not show any geographical or host-specific clustering, and a similar outcome was obtained using population pairwise Fst statistics. These results demonstrated that ITS-2 rDNA does not appear to be suitable for examining genetic diversity among mite populations.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Center for Fish and Wildlife Health (FIWI)

UniBE Contributor:

Ryser, Marie Pierre

Subjects:

600 Technology > 630 Agriculture

ISSN:

0304-4017

Publisher:

Elsevier

Language:

English

Submitter:

Factscience Import

Date Deposited:

04 Oct 2013 15:25

Last Modified:

18 Jul 2014 16:46

Publisher DOI:

10.1016/j.vetpar.2008.10.001

Web of Science ID:

000263308900014

URI:

https://boris.unibe.ch/id/eprint/38347 (FactScience: 221201)

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