Alasaad, S.; Soglia, D.; Spalenza, V.; Maione, S.; Soriguer, R.C.; Perez, J.M.; Rasero, R.; Degiorgis, Marie Pierre; Nimmervoll, H.; Zhu, X.Q.; Rossi, L. (2009). Is ITS-2 rDNA suitable marker for genetic characterization of Sarcoptes mites from different wild animals in different geographic areas? Veterinary parasitology, 159(2), pp. 181-5. Amsterdam: Elsevier 10.1016/j.vetpar.2008.10.001
Full text not available from this repository.The present study examined the relationship among individual Sarcoptes scabiei mites from 13 wild mammalian populations belonging to nine species in four European countries using the second internal transcribed spacer (ITS-2) of nuclear ribosomal DNA (rDNA) as genetic marker. The ITS-2 plus primer flanking 5.8S and 28S rDNA (ITS-2+) was amplified from individual mites by polymerase chain reaction (PCR) and the amplicons were sequenced directly. A total of 148 ITS-2+ sequences of 404bp in length were obtained and 67 variable sites were identified (16.59%). UPGMA analyses did not show any geographical or host-specific clustering, and a similar outcome was obtained using population pairwise Fst statistics. These results demonstrated that ITS-2 rDNA does not appear to be suitable for examining genetic diversity among mite populations.
Item Type: |
Journal Article (Original Article) |
|---|---|
Division/Institute: |
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute for Fish and Wildlife Health (FIWI) |
UniBE Contributor: |
Ryser, Marie Pierre |
Subjects: |
600 Technology > 630 Agriculture |
ISSN: |
0304-4017 |
Publisher: |
Elsevier |
Language: |
English |
Submitter: |
Factscience Import |
Date Deposited: |
04 Oct 2013 15:25 |
Last Modified: |
05 Dec 2022 14:26 |
Publisher DOI: |
10.1016/j.vetpar.2008.10.001 |
Web of Science ID: |
000263308900014 |
URI: |
https://boris.unibe.ch/id/eprint/38347 (FactScience: 221201) |
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