Herold, Marco J.; Rohrbeck, Leona; Lang, Mathias J.; Grumont, Raelene; Gerondakis, Steve; Tai, Lin; Bouillet, Philippe; Kaufmann, Thomas; Strasser, Andreas (2013). Foxo-mediated Bim transcription is dispensable for the apoptosis of hematopoietic cells that is mediated by this BH3-only protein. EMBO REPORTS, 14(11), pp. 992-998. Nature 10.1038/embor.2013.152
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The BH3-only protein Bim is a critical initiator of apoptosis in hematopoietic cells. Bim is upregulated in response to growth factor withdrawal and in vitro studies have implicated the transcription factor Foxo3a as a critical inducer. To test the importance of this regulation in vivo, we generated mice with mutated Foxo-binding sites within the Bim promoters (Bim(ΔFoxo/ΔFoxo)). Contrary to Bim-deficient mice, Bim(ΔFoxo/ΔFoxo) mice had a normal hematopoietic system. Moreover, cytokine-dependent haematopoietic cells from Bim(ΔFoxo/ΔFoxo) and wt mice died at similar rates. These results indicate that regulation of Bim by Foxo transcription factors is not critical for the killing of hematopoietic cells.
Item Type: |
Journal Article (Original Article) |
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Division/Institute: |
04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Pharmacology |
UniBE Contributor: |
Kaufmann, Thomas (B) |
Subjects: |
600 Technology > 610 Medicine & health |
ISSN: |
1469-221X |
Publisher: |
Nature |
Language: |
English |
Submitter: |
Anita Dähler |
Date Deposited: |
06 Dec 2013 03:49 |
Last Modified: |
29 Mar 2023 23:33 |
Publisher DOI: |
10.1038/embor.2013.152 |
PubMed ID: |
24060902 |
Uncontrolled Keywords: |
apoptosis; Bcl-2; BH3-only proteins; Bim; hematopoiesis |
BORIS DOI: |
10.7892/boris.39538 |
URI: |
https://boris.unibe.ch/id/eprint/39538 |