Structures of SAS-6 suggest its organization in centrioles

van Breugel, Mark; Hirono, Masafumi; Andreeva, Antonina; Yanagisawa, Haru-aki; Yamaguchi, Shoko; Nakazawa, Yuki; Morgner, Nina; Petrovich, Miriana; Ebong, Ima-Obong; Robinson, Carol V; Johnson, Christopher M; Veprintsev, Dmitry; Zuber, Benoît (2011). Structures of SAS-6 suggest its organization in centrioles. Science, 331(6021), pp. 1196-9. Washington, D.C.: American Association for the Advancement of Science 10.1126/science.1199325

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Centrioles are cylindrical, ninefold symmetrical structures with peripheral triplet microtubules strictly required to template cilia and flagella. The highly conserved protein SAS-6 constitutes the center of the cartwheel assembly that scaffolds centrioles early in their biogenesis. We determined the x-ray structure of the amino-terminal domain of SAS-6 from zebrafish, and we show that recombinant SAS-6 self-associates in vitro into assemblies that resemble cartwheel centers. Point mutations are consistent with the notion that centriole formation in vivo depends on the interactions that define the self-assemblies observed here. Thus, these interactions are probably essential to the structural organization of cartwheel centers.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Anatomy

UniBE Contributor:

Zuber, Benoît

ISSN:

0036-8075

Publisher:

American Association for the Advancement of Science

Language:

English

Submitter:

Benoît Zuber

Date Deposited:

04 Oct 2013 14:15

Last Modified:

20 Mar 2014 20:22

Publisher DOI:

10.1126/science.1199325

PubMed ID:

21273447

Web of Science ID:

000287971200043

URI:

https://boris.unibe.ch/id/eprint/4092 (FactScience: 208213)

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