van Breugel, Mark; Hirono, Masafumi; Andreeva, Antonina; Yanagisawa, Haru-aki; Yamaguchi, Shoko; Nakazawa, Yuki; Morgner, Nina; Petrovich, Miriana; Ebong, Ima-Obong; Robinson, Carol V; Johnson, Christopher M; Veprintsev, Dmitry; Zuber, Benoît (2011). Structures of SAS-6 suggest its organization in centrioles. Science, 331(6021), pp. 1196-9. Washington, D.C.: American Association for the Advancement of Science 10.1126/science.1199325
Full text not available from this repository.Centrioles are cylindrical, ninefold symmetrical structures with peripheral triplet microtubules strictly required to template cilia and flagella. The highly conserved protein SAS-6 constitutes the center of the cartwheel assembly that scaffolds centrioles early in their biogenesis. We determined the x-ray structure of the amino-terminal domain of SAS-6 from zebrafish, and we show that recombinant SAS-6 self-associates in vitro into assemblies that resemble cartwheel centers. Point mutations are consistent with the notion that centriole formation in vivo depends on the interactions that define the self-assemblies observed here. Thus, these interactions are probably essential to the structural organization of cartwheel centers.
Item Type: |
Journal Article (Original Article) |
|---|---|
Division/Institute: |
04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Anatomy |
UniBE Contributor: |
Zuber, Benoît |
ISSN: |
0036-8075 |
Publisher: |
American Association for the Advancement of Science |
Language: |
English |
Submitter: |
Benoît Zuber |
Date Deposited: |
04 Oct 2013 14:15 |
Last Modified: |
05 Dec 2022 14:03 |
Publisher DOI: |
10.1126/science.1199325 |
PubMed ID: |
21273447 |
Web of Science ID: |
000287971200043 |
URI: |
https://boris.unibe.ch/id/eprint/4092 (FactScience: 208213) |
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