Identification and expression of different dehydrin subclasses involved in the drought response of Trifolium repens

Vaseva, Irina; Anders, Iwona; Feller, Urs (2014). Identification and expression of different dehydrin subclasses involved in the drought response of Trifolium repens. Journal of Plant Physiology, 171(3-4), pp. 213-224. Elsevier 10.1016/j.jplph.2013.07.013

Preview	Text 41796.pdf - Accepted Version Available under License Publisher holds Copyright. Download (355kB) | Preview
	Text vaseva_anders.pdf - Published Version Restricted to registered users only Available under License Publisher holds Copyright. Download (2MB)

Reverse transcribed RNAs coding for YnKn, YnSKn, SKn, and KS dehydrin types in drought-stressed white clover (Trifolium repens) were identified and characterized. The nucleotide analyses revealed the complex nature of dehydrin-coding sequences, often featured with alternative start and stop codons within the open reading frames, which could be a prerequisite for high variability among the transcripts originating from a single gene. For some dehydrin sequences, the existence of natural antisense transcripts was predicted. The differential distribution of dehydrin homologues in roots and leaves from a single white clover stolon under normal and drought conditions was evaluated by semi-quantitative RT-PCR and immunoblots with antibodies against the conserved K-, Y- and S-segments. The data suggest that different dehydrin classes have distinct roles in the drought stress response and vegetative development, demonstrating some specific characteristic features. Substantial levels of YSK-type proteins with different molecular weights were immunodetected in the non-stressed developing leaves. The acidic SK2 and KS dehydrin transcripts exhibited some developmental gradient in leaves. A strong increase of YK transcripts was documented in the fully expanded leaves and roots of drought-stressed individuals. The immunodetected drought-induced signals imply that Y- and K-segment containing dehydrins could be the major inducible Late Embryogenesis Abundant class 2 proteins (LEA 2) that accumulate predominantly under drought.

Interest & Impact

Downloads

551 since deposited on 17 Feb 2014
41 in the past 12 months

Citations

5 Citations in Web of Science ®
6 Citations in Scopus

Search

in Google Scholar™

Services

Actions (login required)

Edit item

Actions (login required)

Edit item