Identification and expression of different dehydrin subclasses involved in the drought response of Trifolium repens

Vaseva, Irina; Anders, Iwona; Feller, Urs (2014). Identification and expression of different dehydrin subclasses involved in the drought response of Trifolium repens. Journal of Plant Physiology, 171(3-4), pp. 213-224. Elsevier 10.1016/j.jplph.2013.07.013

[img]
Preview
Text
41796.pdf - Accepted Version
Available under License Publisher holds Copyright.

Download (355kB) | Preview
[img] Text
vaseva_anders.pdf - Published Version
Restricted to registered users only
Available under License Publisher holds Copyright.

Download (2MB) | Request a copy

Reverse transcribed RNAs coding for YnKn, YnSKn, SKn, and KS dehydrin types in drought-stressed white clover (Trifolium repens) were identified and characterized. The nucleotide analyses revealed the complex nature of dehydrin-coding sequences, often featured with alternative start and stop codons within the open reading frames, which could be a prerequisite for high variability among the transcripts originating from a single gene. For some dehydrin sequences, the existence of natural antisense transcripts was predicted. The differential distribution of dehydrin homologues in roots and leaves from a single white clover stolon under normal and drought conditions was evaluated by semi-quantitative RT-PCR and immunoblots with antibodies against the conserved K-, Y- and S-segments. The data suggest that different dehydrin classes have distinct roles in the drought stress response and vegetative development, demonstrating some specific characteristic features. Substantial levels of YSK-type proteins with different molecular weights were immunodetected in the non-stressed developing leaves. The acidic SK2 and KS dehydrin transcripts exhibited some developmental gradient in leaves. A strong increase of YK transcripts was documented in the fully expanded leaves and roots of drought-stressed individuals. The immunodetected drought-induced signals imply that Y- and K-segment containing dehydrins could be the major inducible Late Embryogenesis Abundant class 2 proteins (LEA 2) that accumulate predominantly under drought.

Item Type:

Journal Article (Original Article)

Division/Institute:

08 Faculty of Science > Department of Biology > Institute of Plant Sciences (IPS) > Plant nutrition [discontinued]
08 Faculty of Science > Department of Biology > Institute of Plant Sciences (IPS)
10 Strategic Research Centers > Oeschger Centre for Climate Change Research (OCCR)

UniBE Contributor:

Vaseva, Irina; Anders, Iwona and Feller, Urs

Subjects:

500 Science > 580 Plants (Botany)

ISSN:

0176-1617

Publisher:

Elsevier

Language:

English

Submitter:

Peter Alfred von Ballmoos-Haas

Date Deposited:

17 Feb 2014 13:57

Last Modified:

13 Dec 2014 07:22

Publisher DOI:

10.1016/j.jplph.2013.07.013

Uncontrolled Keywords:

Dehydrins; Drought; Immunoblot; Splice variants; Trifolium repens

BORIS DOI:

10.7892/boris.41796

URI:

https://boris.unibe.ch/id/eprint/41796

Actions (login required)

Edit item Edit item
Provide Feedback