Structural bases for the interaction and stabilization of the human amino acid transporter LAT2 with its ancillary protein 4F2hc.

Rosell, Albert; Meury, Marcel; Alvarez-Marimon, Elena; Costa Torres, Meritxell; Pérez-Cano, Laura; Zorzano, Antonio; Fernández-Recio, Juan; Palacín, Manuel; Fotiadis, Dimitrios José (2014). Structural bases for the interaction and stabilization of the human amino acid transporter LAT2 with its ancillary protein 4F2hc. Proceedings of the National Academy of Sciences of the United States of America - PNAS, 111(8), pp. 2966-2971. National Academy of Sciences NAS 10.1073/pnas.1323779111

[img] Text
PNAS-2014-Rosell-2966-71.pdf - Published Version
Restricted to registered users only
Available under License Publisher holds Copyright.

Download (1MB) | Request a copy

Heteromeric amino acid transporters (HATs) are the unique example, known in all kingdoms of life, of solute transporters composed of two subunits linked by a conserved disulfide bridge. In metazoans, the heavy subunit is responsible for the trafficking of the heterodimer to the plasma membrane, and the light subunit is the transporter. HATs are involved in human pathologies such as amino acidurias, tumor growth and invasion, viral infection and cocaine addiction. However structural information about interactions between the heavy and light subunits of HATs is scarce. In this work, transmission electron microscopy and single-particle analysis of purified human 4F2hc/L-type amino acid transporter 2 (LAT2) heterodimers overexpressed in the yeast Pichia pastoris, together with docking analysis and crosslinking experiments, reveal that the extracellular domain of 4F2hc interacts with LAT2, almost completely covering the extracellular face of the transporter. 4F2hc increases the stability of the light subunit LAT2 in detergent-solubilized Pichia membranes, allowing functional reconstitution of the heterodimer into proteoliposomes. Moreover, the extracellular domain of 4F2hc suffices to stabilize solubilized LAT2. The interaction of 4F2hc with LAT2 gives insights into the structural bases for light subunit recognition and the stabilizing role of the ancillary protein in HATs.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Biochemistry and Molecular Medicine

UniBE Contributor:

Meury, Marcel; Costa Torres, Meritxell and Fotiadis, Dimitrios José

Subjects:

500 Science > 570 Life sciences; biology
600 Technology > 610 Medicine & health

ISSN:

0027-8424

Publisher:

National Academy of Sciences NAS

Language:

English

Submitter:

Patrizia Catucci

Date Deposited:

23 Jul 2014 16:50

Last Modified:

11 Nov 2015 13:44

Publisher DOI:

10.1073/pnas.1323779111

PubMed ID:

24516142

Uncontrolled Keywords:

4F2hc, ectodomain, CD98hc

BORIS DOI:

10.7892/boris.43404

URI:

https://boris.unibe.ch/id/eprint/43404

Actions (login required)

Edit item Edit item
Provide Feedback