Evaluation of PCR electrospray-ionization mass spectrometry for rapid molecular diagnosis of bovine mastitis

Perreten, Vincent; Endimiani, Andrea; Thomann, Andreas; Wipf, Juliette R. K.; Rossano, Alexandra; Bodmer, Michèle; Raemy, Andreas; Sannes-Lowery, Kristin A.; Ecker, David J.; Sampath, Rangarajan; Bonomo, Robert A. (2013). Evaluation of PCR electrospray-ionization mass spectrometry for rapid molecular diagnosis of bovine mastitis. Journal of dairy science, 96(6), pp. 3611-3620. American Dairy Science Association 10.3168/jds.2012-6124

[img]
Preview
Text
1-s2.0-S002203021300283X-main.pdf - Published Version
Available under License Publisher holds Copyright.

Download (165kB) | Preview

Bovine mastitis, an inflammatory disease of the mammary gland, is one of the most costly diseases affecting the dairy industry. The treatment and prevention of this disease is linked heavily to the use of antibiotics in agriculture and early detection of the primary pathogen is essential to control the disease. Milk samples (n=67) from cows suffering from mastitis were analyzed for the presence of pathogens using PCR electrospray-ionization mass spectrometry (PCR/ESI-MS) and were compared with standard culture diagnostic methods. Concurrent identification of the primary mastitis pathogens was obtained for 64% of the tested milk samples, whereas divergent results were obtained for 27% of the samples. The PCR/ESI-MS failed to identify some of the primary pathogens in 18% of the samples, but identified other pathogens as well as microorganisms in samples that were negative by culture. The PCR/ESI-MS identified bacteria to the species level as well as yeasts and molds in samples that contained a mixed bacterial culture (9%). The sensitivity of the PCR/ESI-MS for the most common pathogens ranged from 57.1 to 100% and the specificity ranged from 69.8 to 100% using culture as gold standard. The PCR/ESI-MS also revealed the presence of the methicillin-resistant gene mecA in 16.2% of the milk samples, which correlated with the simultaneous detection of staphylococci including Staphylococcus aureus. We demonstrated that PCR/ESI-MS, a more rapid diagnostic platform compared with bacterial culture, has the significant potential to serve as an important screening method in the diagnosis of bovine clinical mastitis and has the capacity to be used in infection control programs for both subclinical and clinical disease.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Research Foci > Veterinary Public Health / Herd Health Management
05 Veterinary Medicine > Research Foci > Host-Pathogen Interaction
04 Faculty of Medicine > Service Sector > Institute for Infectious Diseases
05 Veterinary Medicine > Department of Clinical Veterinary Medicine (DKV)
05 Veterinary Medicine > Department of Clinical Veterinary Medicine (DKV) > Clinic for Ruminants
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP)
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Veterinary Bacteriology

UniBE Contributor:

Perreten, Vincent; Endimiani, Andrea; Thomann, Andreas; Wipf, Juliette Ramona Karin; Rossano, Alexandra; Bodmer, Michèle and Raemy, Andreas

Subjects:

500 Science > 570 Life sciences; biology
600 Technology > 610 Medicine & health
600 Technology > 630 Agriculture

ISSN:

0022-0302

Publisher:

American Dairy Science Association

Language:

English

Submitter:

Susanne Portner

Date Deposited:

02 May 2014 08:52

Last Modified:

02 Mar 2015 15:04

Publisher DOI:

10.3168/jds.2012-6124

PubMed ID:

23587378

Uncontrolled Keywords:

milk, detection, dairy cow, method

BORIS DOI:

10.7892/boris.43818

URI:

https://boris.unibe.ch/id/eprint/43818

Actions (login required)

Edit item Edit item
Provide Feedback