Nano-stenciled RGD-gold patterns that inhibit focal contact maturation induce lamellipodia formation in fibroblasts

Lutz, Roman; Pataky, Kristopher; Gadhari, Neha; Marelli, Mattia; Brugger, Juergen; Chiquet, Matthias (2011). Nano-stenciled RGD-gold patterns that inhibit focal contact maturation induce lamellipodia formation in fibroblasts. PLoS ONE, 6(9), e25459. Lawrence, Kans.: Public Library of Science 10.1371/journal.pone.0025459

[img]
Preview
Text
journal.pone.0025459.pdf - Published Version
Available under License Creative Commons: Attribution (CC-BY).

Download (1MB) | Preview

Cultured fibroblasts adhere to extracellular substrates by means of cell-matrix adhesions that are assembled in a hierarchical way, thereby gaining in protein complexity and size. Here we asked how restricting the size of cell-matrix adhesions affects cell morphology and behavior. Using a nanostencil technique, culture substrates were patterned with gold squares of a width and spacing between 250 nm and 2 µm. The gold was functionalized with RGD peptide as ligand for cellular integrins, and mouse embryo fibroblasts were plated. Limiting the length of cell-matrix adhesions to 500 nm or less disturbed the maturation of vinculin-positive focal complexes into focal contacts and fibrillar adhesions, as indicated by poor recruitment of ?5-integrin. We found that on sub-micrometer patterns, fibroblasts spread extensively, but did not polarize. Instead, they formed excessive numbers of lamellipodia and a fine actin meshwork without stress fibers. Moreover, these cells showed aberrant fibronectin fibrillogenesis, and their speed of directed migration was reduced significantly compared to fibroblasts on 2 µm square patterns. Interference with RhoA/ROCK signaling eliminated the pattern-dependent differences in cell morphology. Our results indicate that manipulating the maturation of cell-matrix adhesions by nanopatterned surfaces allows to influence morphology, actin dynamics, migration and ECM assembly of adhering fibroblasts.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > School of Dental Medicine > Department of Orthodontics

UniBE Contributor:

Gadhari, Neha and Chiquet, Matthias

ISSN:

1932-6203

Publisher:

Public Library of Science

Language:

English

Submitter:

Eveline Carmen Schuler

Date Deposited:

04 Oct 2013 14:16

Last Modified:

25 Jan 2017 12:16

Publisher DOI:

10.1371/journal.pone.0025459

PubMed ID:

21980465

Web of Science ID:

000295933700038

BORIS DOI:

10.7892/boris.4458

URI:

https://boris.unibe.ch/id/eprint/4458 (FactScience: 208673)

Actions (login required)

Edit item Edit item
Provide Feedback