Insights into the regulation of GPEET procyclin during differentiation from early to late procyclic forms of Trypanosoma brucei.

Knüsel, Sebastian; Roditi, Isabel (2013). Insights into the regulation of GPEET procyclin during differentiation from early to late procyclic forms of Trypanosoma brucei. Molecular and biochemical parasitology, 191(2), pp. 66-74. Elsevier 10.1016/j.molbiopara.2013.09.004

[img]
Preview
Text
Knüsel_2013.pdf - Published Version
Available under License BORIS Standard License.

Download (2MB) | Preview

The procyclic form of Trypanosoma brucei colonises the gut of its insect vector, the tsetse fly. GPEET and EP procyclins constitute the parasite's surface coat at this stage of the life cycle, and the presence or absence of GPEET distinguishes between early and late procyclic forms, respectively. Differentiation from early to late procyclic forms in vivo occurs in the fly midgut and can be mimicked in culture. Our analysis of this transition in vitro delivered new insights into the process of GPEET repression. First, we could show that parasites followed a concrete sequence of events upon triggering differentiation: after undergoing an initial growth arrest, cells lost GPEET protein, and finally late procyclic forms resumed proliferation. Second, we determined the stability of both GPEET and EP mRNA during differentiation. GPEET mRNA is exceptionally stable in early procyclic forms, with a half-life >6h. The GPEET mRNA detected in late procyclic form cultures is a mixture of transcripts from both bona fide late procyclic forms and GPEET-positive 'laggard' parasites present in these cultures. However, its stability was clearly reduced during differentiation and in late procyclic form cultures. Alternatively processed GPEET transcripts were enriched in samples from late procyclic forms, suggesting that altered mRNA processing might contribute to repression of GPEET in this developmental stage. In addition, we detected GPEET transcripts with non-templated oligo(U) tails that were enriched in late procyclic forms. To the best of our knowledge, this is the first study reporting a uridylyl-tailed, nuclear-encoded mRNA species in trypanosomatids or any other protozoa.

Item Type:

Journal Article (Original Article)

Division/Institute:

08 Faculty of Science > Department of Biology > Institute of Cell Biology

UniBE Contributor:

Knüsel, Sebastian and Roditi, Isabel

Subjects:

500 Science > 570 Life sciences; biology

ISSN:

0166-6851

Publisher:

Elsevier

Funders:

[4] Swiss National Science Foundation
[UNSPECIFIED] Howard Hughes Medical Institute, USA

Language:

English

Submitter:

Isabel Roditi

Date Deposited:

12 Apr 2014 10:26

Last Modified:

05 Dec 2014 11:19

Publisher DOI:

10.1016/j.molbiopara.2013.09.004

PubMed ID:

24076427

BORIS DOI:

10.7892/boris.45160

URI:

https://boris.unibe.ch/id/eprint/45160

Actions (login required)

Edit item Edit item
Provide Feedback