Lack of Host Gut Microbiota Alters Immune Responses and Intestinal Granuloma Formation during Schistosomiasis

Holzscheiter, Maria; Layland, Laura E.; Loffredo-Verde, Eva; Mair, Katrin; Vogelmann, Roger; Langer, Rupert; Wagner, Hermann; Prazeres da Costa, Clarissa (2014). Lack of Host Gut Microbiota Alters Immune Responses and Intestinal Granuloma Formation during Schistosomiasis. Clinical and experimental immunology, 175(2), pp. 246-257. Blackwell Scientific Publications 10.1111/cei.12230

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Fatalities from schistosome infections arise due to granulomatous, immune-mediated responses to eggs that become trapped in host tissues. Schistosome-specific immune responses are characterized by initial Th1 responses and our previous studies demonstrated that Myd88-deficient mice failed to initiate such responses in vivo. Paradoxically, schistosomal antigens fail to stimulate innate cells to release pro-inflammatory cytokines in vitro. Since S. mansoni infection is an intestinal disease, we hypothesized that commensal bacteria could act as bystander activators of the intestinal innate immune system to instigate Th1 responses. Using a broad spectrum of orally-administered antibiotics and antimycotics we analyzed schistosome-infected mice that were simultaneously depleted of gut bacteria. After depletion there was significantly less inflammation in the intestine which was accompanied by decreased intestinal granuloma development. In contrast, liver pathology remained unaltered. In addition, schistosome-specific immune responses were skewed and fecal egg excretion was diminished. This study demonstrates that host microbiota can act as a third partner in instigating helminth-specific immune responses.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Service Sector > Institute of Pathology

UniBE Contributor:

Langer, Rupert

Subjects:

500 Science > 570 Life sciences; biology
600 Technology > 610 Medicine & health

ISSN:

0009-9104

Publisher:

Blackwell Scientific Publications

Language:

English

Submitter:

Andrea Arnold

Date Deposited:

02 Apr 2014 15:55

Last Modified:

10 Sep 2015 09:32

Publisher DOI:

10.1111/cei.12230

PubMed ID:

24168057

Uncontrolled Keywords:

Host-parasite interaction, Immunopathology, Schistosomiasis, Th responses, gut-microbiota

BORIS DOI:

10.7892/boris.45915

URI:

https://boris.unibe.ch/id/eprint/45915

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