The need for transparency and good practices in the qPCR literature

Bustin, Stephen A.; Benes, Vladimir; Garson, Jeremy; Hellemans, Jan; Huggett, Jim; Kubista, Mikael; Mueller, Reinhold; Nolan, Tania; Pfaffl, Michael W.; Shipley, Gregory; Wittwer, Carl T.; Schjerling, Peter; Day, Philip J.; Abreu, Mónica; Aguado, Begoña; Beaulieu, Jean-François; Beckers, Anneleen; Bogaert, Sara; Browne, John A.; Carrasco-Ramiro, Fernando; ... (2013). The need for transparency and good practices in the qPCR literature. Nature methods, 10(11), pp. 1063-1067. Nature Publishing Group 10.1038/nmeth.2697

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Two surveys of over 1,700 publications whose authors use quantitative real-time PCR (qPCR) reveal a lack of transparent and comprehensive reporting of essential technical information. Reporting standards are significantly improved in publications that cite the Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines, although such publications are still vastly outnumbered by those that do not.

Item Type:

Journal Article (Further Contribution)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > Forschungsbereich Pathologie > Forschungsgruppe Molekularbiologie
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR)

UniBE Contributor:

Jaggi, Rolf

Subjects:

600 Technology > 610 Medicine & health

ISSN:

1548-7091

Publisher:

Nature Publishing Group

Language:

English

Submitter:

Verena de Serra Frazao-Bill

Date Deposited:

21 May 2014 11:27

Last Modified:

21 May 2014 11:27

Publisher DOI:

10.1038/nmeth.2697

PubMed ID:

24173381

URI:

https://boris.unibe.ch/id/eprint/46005

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