The expression levels of three raft-associated molecules in cultivated vascular cells are dependent on culture conditions

Monastyrskaya, K.; Babiychuk, E. B.; Schittny, J. C.; Rescher, U.; Gerke, V.; Mannherz, H.-G.; Draeger, A. (2003). The expression levels of three raft-associated molecules in cultivated vascular cells are dependent on culture conditions. Cellular and molecular life sciences, 60(12), pp. 2702-2709. Birkhäuser-Verlag 10.1007/s00018-003-3307-1

Preview

Text art%3A10.1007%2Fs00018-003-3307-1.pdf - Published Version Available under License Publisher holds Copyright. Download (337kB) | Preview

Relaying a signal across the plasma membrane requires functional connections between the partner molecules. Membrane microdomains or lipid rafts provide an environment in which such specific interactions can take place. The integrity of these sites is often taken for granted when signalling pathways are investigated in cell culture. However, it is well known that smooth muscle and endothelial cells undergo cytoskeletal rearrangements during monolayer culturing. Likewise affected--and with potentially important consequences for signalling events--is the organization of the plasma membrane. The expression levels of three raft markers were massively upregulated, and raft-associated 5'-nucleotidase activity increased in conventional monolayer cultures as compared with a spheroidal coculture model, shown to promote the differentiation of endothelial cells. Our data point to a shift of raft components in monolayer cultures and demonstrate potential advantages of the spheroid coculture system for investigation of raft-mediated signalling events in endothelial cells.

Interest & Impact

Downloads

221 since deposited on 01 Sep 2014
27 in the past 12 months

Citations

5 Citations in Web of Science ®
6 Citations in Scopus

Search

in Google Scholar™

Services

Actions (login required)

Edit item

Actions (login required)

Edit item