The expression levels of three raft-associated molecules in cultivated vascular cells are dependent on culture conditions

Monastyrskaya, K.; Babiychuk, E. B.; Schittny, J. C.; Rescher, U.; Gerke, V.; Mannherz, H.-G.; Draeger, A. (2003). The expression levels of three raft-associated molecules in cultivated vascular cells are dependent on culture conditions. Cellular and molecular life sciences, 60(12), pp. 2702-2709. Birkhäuser-Verlag 10.1007/s00018-003-3307-1

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Relaying a signal across the plasma membrane requires functional connections between the partner molecules. Membrane microdomains or lipid rafts provide an environment in which such specific interactions can take place. The integrity of these sites is often taken for granted when signalling pathways are investigated in cell culture. However, it is well known that smooth muscle and endothelial cells undergo cytoskeletal rearrangements during monolayer culturing. Likewise affected--and with potentially important consequences for signalling events--is the organization of the plasma membrane. The expression levels of three raft markers were massively upregulated, and raft-associated 5'-nucleotidase activity increased in conventional monolayer cultures as compared with a spheroidal coculture model, shown to promote the differentiation of endothelial cells. Our data point to a shift of raft components in monolayer cultures and demonstrate potential advantages of the spheroid coculture system for investigation of raft-mediated signalling events in endothelial cells.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Anatomy
04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Anatomy > Functional Anatomy
04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Anatomy > Cell Biology

UniBE Contributor:

Monastyrskaya-Stäuber, Katia; Babiychuk, Eduard; Schittny, Johannes and Draeger, Annette

Subjects:

600 Technology > 610 Medicine & health

ISSN:

1420-682X

Publisher:

Birkhäuser-Verlag

Language:

English

Submitter:

Johannes Schittny

Date Deposited:

01 Sep 2014 11:53

Last Modified:

23 Oct 2019 11:24

Publisher DOI:

10.1007/s00018-003-3307-1

PubMed ID:

14685693

Uncontrolled Keywords:

Signalling, smooth muscle, endothelial cells, 5′-nucleotidase, annexin

BORIS DOI:

10.7892/boris.50149

URI:

https://boris.unibe.ch/id/eprint/50149

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