Phagocytic uptake of Encephalitozoon cuniculi by nonprofessional phagocytes

Couzinet, Sabine; Cejas, Elisabeth; Schittny, Johannes; Deplazes, Peter; Weber, Rainer; Zimmerli, Stefan (2000). Phagocytic uptake of Encephalitozoon cuniculi by nonprofessional phagocytes. Infection and immunity, 68(12), pp. 6939-6945. American Society for Microbiology 10.1128/IAI.68.12.6939-6945.2000

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Encephalitozoon cuniculi is an obligate intracellular, spore-forming parasite belonging to the microsporidia that can cause disseminated infection in immunocompromised persons. E. cuniculi spores infect host cells by germination, i.e., by explosively everting the polar filament, through which the spore contents (sporoplasms) are subsequently injected into the cytoplasm. In addition, we observed intracellular, nongerminated spores in various nonprofessional phagocytes. In MRC5 cells, the number of internalized spores was approximately 10-fold higher than the number of injected sporoplasms. Compared to the rate of uptake by human monocyte-derived macrophages, internalization rates by A549 cells, MRC5 cells, and 293 cells were 0.6, 4.4, and 22.2%, respectively. The mechanism of uptake was studied in MRC5 cells. Killed spores were internalized at the same rate as live spores, indicating that nongerminated parasites do not actively participate in cell entry. Cytochalasin D inhibited uptake of spores by 95%, demonstrating an actin-dependent process. By electron and epifluorescence microscopy, intracellular spores were found in a tightly fitting membrane-bound compartment. The vacuole containing the spores was positive for the lysosomal membrane protein LAMP-1 and colocalized with the late endosomal-lysosomal content marker rhodamine dextran. Our results show that, in addition to the unique way in which microsporidia infect cells, E. cuniculi spores enter nonprofessional phagocytes by phagocytosis and traffic into a late endosomal-lysosomal compartment.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Service Sector > Institute for Infectious Diseases
04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Anatomy > Functional Anatomy

UniBE Contributor:

Couzinet, Sabine; Schittny, Johannes and Zimmerli, Stefan

Subjects:

500 Science > 570 Life sciences; biology
600 Technology > 610 Medicine & health

ISSN:

0019-9567

Publisher:

American Society for Microbiology

Language:

English

Submitter:

Johannes Schittny

Date Deposited:

25 Aug 2014 14:24

Last Modified:

19 Dec 2014 17:16

Publisher DOI:

10.1128/IAI.68.12.6939-6945.2000

PubMed ID:

11083817

BORIS DOI:

10.7892/boris.50158

URI:

https://boris.unibe.ch/id/eprint/50158

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