Protein cross-linking mediated by tissue transglutaminase correlates with the maturation of extracellular matrices during lung development

Schittny, Johannes C.; Paulsson, Mats; Vallan, Claudio; Burri, Peter H.; Kedei, Noemi; Aeschlimann, Daniel (1997). Protein cross-linking mediated by tissue transglutaminase correlates with the maturation of extracellular matrices during lung development. American journal of respiratory cell and molecular biology, 17(3), pp. 334-343. American Lung Association 10.1165/ajrcmb.17.3.2737

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At birth, the mammalian lung is still immature. The alveoli are not yet formed and the interairspace walls contain two capillary layers which are separated by an interstitial core. After alveolarization (first 2 postnatal weeks in rats) the alveolar septa mature: their capillary layers merge, the amount of connective tissue decreases, and the mature lung parenchyma is formed (second and third week). During the first 3 wk of life the role of tissue transglutaminase (tTG) was studied in rat lung by immunostaining of cryostat and paraffin sections, by Northern and Western blotting, and by a quantitative determination of gamma-glutamyl-epsilon-lysine. While enzyme activity and intracellular tTG were already present before term, the enzyme product (gamma-glutamyl-epsilon-lysine-crosslink) and extracellular tTG appeared between postnatal days 10 and 19 in the lung parenchyma. In large blood vessels and large airways, which mature earlier than the parenchyma, both the enzyme product and extracellular tTG had already appeared at the end of the first postnatal week. We conclude that tTG is expressed and externalized into the extracellular matrix of lung shortly before maturation of an organ area. Because tTG covalently and irreversibly crosslinks extracellular matrix proteins, we hypothesize that it may prevent or delay further remodeling of basement membranes and may stabilize other extracellular components, such as microfibrils.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Service Sector > Institute of Pathology
04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Anatomy
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DCR Services > Core Facility Zytometrie-Labor/FACSlab
04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Anatomy > Functional Anatomy

UniBE Contributor:

Schittny, Johannes; Vallan, Claudio and Burri, Peter Hermann

Subjects:

500 Science > 570 Life sciences; biology
600 Technology > 610 Medicine & health

ISSN:

1044-1549

Publisher:

American Lung Association

Language:

English

Submitter:

Johannes Schittny

Date Deposited:

25 Aug 2014 10:36

Last Modified:

24 Dec 2014 20:14

Publisher DOI:

10.1165/ajrcmb.17.3.2737

PubMed ID:

9308920

BORIS DOI:

10.7892/boris.50162

URI:

https://boris.unibe.ch/id/eprint/50162

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