The equine DNAH3 gene: SNP discovery and exclusion of an involvement in recurrent airway obstruction (RAO) in European Warmblood horses

Shakhsi Niaei, Mostafa; Klukowska-Rötzler, Jolanta; Drögemüller, Cord; Gerber, Vinzenz; Leeb, Tosso (2013). The equine DNAH3 gene: SNP discovery and exclusion of an involvement in recurrent airway obstruction (RAO) in European Warmblood horses. Archiv Tierzucht / Archives animal breeding, 56(1), pp. 1-10. Leibniz Institute for Farm Animal Biology FBN 10.7482/0003-9438-56-001

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Recurrent airway obstruction is one of the most common airway diseases affecting mature horses. Increased bronchoalveolar mucus, neutrophil accumulation in airways, and airway obstruction are the main features of this disease. Mucociliary clearance is a key component of pulmonary defense mechanisms. Cilia are the motile part of this system and a complex linear array of dynein motors is responsible for their motility by moving along the microtubules in the axonemes of cilia and flagella. We previously detected a QTL for RAO on ECA 13 in a half-sib family of European Warmblood horses. The gene encoding DNAH3 is located in the peak of the detected QTL and encodes a dynein subunit. Therefore, we analysed this gene as a positional and functional candidate gene for RAO. In a mutation analysis of all 62 exons we detected 53 new polymorphisms including 7 non-synonymous variants. We performed an association study using 38 polymorphisms in a cohort of 422 animals. However, after correction for multiple testing we did not detect a significant association of any of these polymorphisms with RAO (P>0.05). Therefore, it seems unlikely that variants at the DNAH3 gene are responsible for the RAO QTL in European Warmblood horses.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Research Foci > Host-Pathogen Interaction
05 Veterinary Medicine > Department of Clinical Veterinary Medicine (DKV) > Equine Clinic
05 Veterinary Medicine > Department of Clinical Veterinary Medicine (DKV)
05 Veterinary Medicine > Department of Clinical Research and Veterinary Public Health (DCR-VPH) > Institute of Genetics
05 Veterinary Medicine > Department of Clinical Research and Veterinary Public Health (DCR-VPH)

UniBE Contributor:

Klukowska, Jolanta; Drögemüller, Cord; Gerber, Vinzenz and Leeb, Tosso

Subjects:

500 Science > 590 Animals (Zoology)
600 Technology > 630 Agriculture

ISSN:

0003-9438

Publisher:

Leibniz Institute for Farm Animal Biology FBN

Language:

English

Submitter:

Susanne Portner

Date Deposited:

28 Jul 2014 11:22

Last Modified:

11 Feb 2015 14:14

Publisher DOI:

10.7482/0003-9438-56-001

Uncontrolled Keywords:

horse, allergy, recurrent airway obstruction, dynein, association

BORIS DOI:

10.7892/boris.52101

URI:

https://boris.unibe.ch/id/eprint/52101

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