Application of real-time fluorescent PCR for quantitative assessment of Neospora caninum infections in organotypic slice cultures of rat central nervous system tissue

Müller, Norbert; Vonlaufen, Nathalie Françoise F.; Gianinazzi, Christian; Leib, Stephen L.; Hemphill, Andrew (2002). Application of real-time fluorescent PCR for quantitative assessment of Neospora caninum infections in organotypic slice cultures of rat central nervous system tissue. Journal of clinical microbiology, 40(1), pp. 252-255. American Society for Microbiology 10.1128/JCM.40.1.252-255.2002

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The previously described Nc5-specific PCR test for the diagnosis of Neospora caninum infections was used to develop a quantitative PCR assay which allows the determination of infection intensities within different experimental and diagnostic sample groups. The quantitative PCR was performed by using a dual fluorescent hybridization probe system and the LightCycler Instrument for online detection of amplified DNA. This assay was successfully applied for demonstrating the parasite proliferation kinetics in organotypic slice cultures of rat brain which were infected in vitro with N. caninum tachyzoites. This PCR-based method of parasite quantitation with organotypic brain tissue samples can be regarded as a novel ex vivo approach for exploring different aspects of cerebral N. caninum infection.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Parasitology
04 Faculty of Medicine > Service Sector > Institute for Infectious Diseases

UniBE Contributor:

Müller, Norbert; Vonlaufen, Nathalie Françoise F.; Gianinazzi, Christian and Leib, Stephen

Subjects:

600 Technology > 630 Agriculture
500 Science > 570 Life sciences; biology
600 Technology > 610 Medicine & health

ISSN:

0095-1137

Publisher:

American Society for Microbiology

Language:

English

Submitter:

Stephen Leib

Date Deposited:

01 Sep 2014 10:13

Last Modified:

25 Dec 2014 11:52

Publisher DOI:

10.1128/JCM.40.1.252-255.2002

PubMed ID:

11773124

BORIS DOI:

10.7892/boris.52756

URI:

https://boris.unibe.ch/id/eprint/52756

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