A differential concentration-dependent effect of IVIg on neutrophil functions: relevance for anti-microbial and anti-inflammatory mechanisms

Casulli, Sarah; Topçu, Selma; Fattoum, Lakhdar; von Gunten, Stephan; Simon, Hans-Uwe; Teillaud, Jean-Luc; Bayry, Jagadeesh; Kaveri, Srini V.; Elbim, Carole (2011). A differential concentration-dependent effect of IVIg on neutrophil functions: relevance for anti-microbial and anti-inflammatory mechanisms. PLoS ONE, 6(10), e26469. Lawrence, Kans.: Public Library of Science 10.1371/journal.pone.0026469

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Background

Polymorphonuclear neutrophils (PMN) play a key role in host defences against invading microorganisms but can also potentiate detrimental inflammatory reactions in case of excessive or misdirected responses. Intravenous immunoglobulins (IVIg) are used to treat patients with immune deficiencies and, at higher doses, in autoimmune, allergic and systemic inflammatory disorders.
Methodology/Principal Findings

We used flow cytometry to examine the effects of IVIg on PMN functions and survival, using whole-blood conditions in order to avoid artifacts due to isolation procedures. IVIg at low concentrations induced PMN activation, as reflected by decreased L-selectin and increased CD11b expression at the PMN surface, oxidative burst enhancement, and prolonged cell survival. In contrast, IVIg at higher concentrations inhibited LPS-induced CD11b degranulation and oxidative burst priming, and counteracted LPS-induced PMN lifespan prolongation.
Conclusions/Significance

IVIg appears to have differential, concentration-dependent effects on PMN, possibly supporting the use of IVIg as either an anti-microbial or an anti-inflammatory agent.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Pharmacology

UniBE Contributor:

von Gunten, Stephan and Simon, Hans-Uwe

ISSN:

1932-6203

Publisher:

Public Library of Science

Language:

English

Submitter:

Factscience Import

Date Deposited:

04 Oct 2013 14:18

Last Modified:

28 Nov 2020 02:19

Publisher DOI:

10.1371/journal.pone.0026469

PubMed ID:

22065996

Web of Science ID:

000296916000012

BORIS DOI:

10.7892/boris.5463

URI:

https://boris.unibe.ch/id/eprint/5463 (FactScience: 210210)

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