Increased FOXP3 expression in tumour-associated tissues of horses affected with equine sarcoid disease.

Mählmann, Kathrin; Hamza, Eman; Marti, Eliane Isabelle; Dolf, Gaudenz; Klukowska, Jolanta; Gerber, Vinzenz; Koch, Christoph (2014). Increased FOXP3 expression in tumour-associated tissues of horses affected with equine sarcoid disease. Veterinary journal, 202(3), pp. 516-521. Elsevier 10.1016/j.tvjl.2014.09.003

[img] Text
Increased FOXP3 expression in tumour associated tissues of horses affected with equine sarcoid disease.pdf - Published Version
Restricted to registered users only
Available under License Publisher holds Copyright.

Download (618kB) | Request a copy

Recent studies suggest that regulatory T cells (Tregs) are associated with disease severity and progression in papilloma virus induced neoplasia. Bovine papilloma virus (BPV) is recognised as the most important aetiological factor in equine sarcoid (ES) disease. The aim of this study was to compare expression levels of Treg markers and associated cytokines in tissue samples of ES-affected equids with skin samples of healthy control horses. Eleven ES-affected, and 12 healthy horses were included in the study. Expression levels of forkhead box protein 3 (FOXP3), interleukin 10 (IL10), interleukin 4 (IL4) and interferon gamma (IFNG) mRNA in lesional and tumour-distant samples from ES-affected horses, as well as in dermal samples of healthy control horses were measured using quantitative reverse transcription polymerase chain reaction (PCR). Expression levels were compared between lesional and tumour-distant as well as between tumour-distant and control samples. Furthermore, BPV-1 E5 DNA in samples of ES-affected horses was quantified using quantitative PCR, and possible associations of viral load, disease severity and gene expression levels were evaluated. Expression levels of FOXP3, IL10 and IFNG mRNA and BPV-1 E5 copy numbers were significantly increased in lesional compared to tumour-distant samples. There was no difference in FOXP3 and cytokine expression in tumour-distant samples from ES- compared with control horses. In tumour-distant samples viral load was positively correlated with IL10 expression and severity score. The increased expression of Treg markers in tumour-associated tissues of ES-affected equids indicates a local, Treg-induced immune suppression.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Research Foci > DermFocus
05 Veterinary Medicine > Research Foci > Host-Pathogen Interaction
05 Veterinary Medicine > Department of Clinical Veterinary Medicine (DKV) > Equine Clinic
05 Veterinary Medicine > Department of Clinical Veterinary Medicine (DKV)
05 Veterinary Medicine > Department of Clinical Research and Veterinary Public Health (DCR-VPH) > Experimental Clinical Research
05 Veterinary Medicine > Department of Clinical Research and Veterinary Public Health (DCR-VPH) > Institute of Genetics

UniBE Contributor:

Mählmann, Kathrin; Hamza, Eman; Marti, Eliane Isabelle; Dolf, Gaudenz; Klukowska, Jolanta; Gerber, Vinzenz and Koch, Christoph

Subjects:

600 Technology > 630 Agriculture
500 Science > 590 Animals (Zoology)

ISSN:

1090-0233

Publisher:

Elsevier

Language:

English

Submitter:

Andrea Gassmann-Suter

Date Deposited:

21 Nov 2014 15:11

Last Modified:

15 Oct 2015 08:54

Publisher DOI:

10.1016/j.tvjl.2014.09.003

PubMed ID:

25266649

Uncontrolled Keywords:

BPV, Equine sarcoid, FOXP3, Horse, Regulatory T cells

BORIS DOI:

10.7892/boris.60123

URI:

https://boris.unibe.ch/id/eprint/60123

Actions (login required)

Edit item Edit item
Provide Feedback