Engineered liposomes sequester bacterial exotoxins and protect from severe invasive infections in mice

Babiychuk, Eduard; Draeger, Annette; Wolfmeier, Heidi Annemarie; Luginbühl, Miriam (2014). Engineered liposomes sequester bacterial exotoxins and protect from severe invasive infections in mice. Nature biotechnology, 33(1), pp. 81-88. Nature America 10.1038/nbt.3037

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Gram-positive bacterial pathogens that secrete cytotoxic pore-forming toxins, such as Staphylococcus aureus and Streptococcus pneumoniae, cause a substantial burden of disease. Inspired by the principles that govern natural toxin-host interactions, we have engineered artificial liposomes that are tailored to effectively compete with host cells for toxin binding. Liposome-bound toxins are unable to lyse mammalian cells in vitro. We use these artificial liposomes as decoy targets to sequester bacterial toxins that are produced during active infection in vivo. Administration of artificial liposomes within 10 h after infection rescues mice from septicemia caused by S. aureus and S. pneumoniae, whereas untreated mice die within 24-33 h. Furthermore, liposomes protect mice against invasive pneumococcal pneumonia. Composed exclusively of naturally occurring lipids, tailored liposomes are not bactericidal and could be used therapeutically either alone or in conjunction with antibiotics to combat bacterial infections and to minimize toxin-induced tissue damage that occurs during bacterial clearance

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Biochemistry and Molecular Medicine
04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Anatomy
04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Anatomy > Cell Biology

UniBE Contributor:

Babiychuk, Eduard; Draeger, Annette and Wolfmeier, Heidi Annemarie

Subjects:

500 Science > 570 Life sciences; biology
600 Technology > 610 Medicine & health

ISSN:

1087-0156

Publisher:

Nature America

Language:

English

Submitter:

Annette Draeger

Date Deposited:

06 Jan 2015 17:35

Last Modified:

28 Apr 2016 08:59

Publisher DOI:

10.1038/nbt.3037

PubMed ID:

25362245

BORIS DOI:

10.7892/boris.61430

URI:

https://boris.unibe.ch/id/eprint/61430

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