Rapid and accurate identification of Escherichia coli K-12 strains

Kuhnert, Peter; Nicolet, Jacques; Frey, Joachim (1995). Rapid and accurate identification of Escherichia coli K-12 strains. Applied and environmental microbiology, 61(11), pp. 4135-4139. American Society for Microbiology

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A specific PCR for the identification of K-12 strains, based on the genetic structure of the O-antigen gene cluster (rfb) of Escherichia coli K-12, is described. The assay clearly differentiates E. coli K-12-derived strains from other E. coli strains used in the laboratory or isolated from human and animal clinical specimens, from food, or from environmental samples. Moreover, lineages of K-12 strains can be distinguished with a second PCR based on the same gene cluster. The method presents a useful tool in identifying K-12 for monitoring strains which are used as biologically safe vehicles in biotechnological research, development, and production processes.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP)
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Veterinary Bacteriology
05 Veterinary Medicine > Other Institutions > Emeriti, Vetsuisse Faculty

UniBE Contributor:

Kuhnert, Peter; Nicolet, Jacques and Frey, Joachim

Subjects:

600 Technology > 630 Agriculture
500 Science > 570 Life sciences; biology

ISSN:

0099-2240

Publisher:

American Society for Microbiology

Language:

English

Submitter:

Peter Kuhnert-Ryser

Date Deposited:

09 Feb 2015 12:46

Last Modified:

09 Feb 2015 12:46

PubMed ID:

8526531

BORIS DOI:

10.7892/boris.62554

URI:

https://boris.unibe.ch/id/eprint/62554

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