Kuhnert, Peter; Nicolet, Jacques; Frey, Joachim (1995). Rapid and accurate identification of Escherichia coli K-12 strains. Applied and environmental microbiology, 61(11), pp. 4135-4139. American Society for Microbiology
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A specific PCR for the identification of K-12 strains, based on the genetic structure of the O-antigen gene cluster (rfb) of Escherichia coli K-12, is described. The assay clearly differentiates E. coli K-12-derived strains from other E. coli strains used in the laboratory or isolated from human and animal clinical specimens, from food, or from environmental samples. Moreover, lineages of K-12 strains can be distinguished with a second PCR based on the same gene cluster. The method presents a useful tool in identifying K-12 for monitoring strains which are used as biologically safe vehicles in biotechnological research, development, and production processes.
Item Type: |
Journal Article (Original Article) |
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Division/Institute: |
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) 05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Veterinary Bacteriology 05 Veterinary Medicine > Other Institutions > Emeriti, Vetsuisse Faculty |
UniBE Contributor: |
Kuhnert, Peter, Nicolet, Jacques, Frey, Joachim |
Subjects: |
600 Technology > 630 Agriculture 500 Science > 570 Life sciences; biology |
ISSN: |
0099-2240 |
Publisher: |
American Society for Microbiology |
Language: |
English |
Submitter: |
Peter Kuhnert-Ryser |
Date Deposited: |
09 Feb 2015 12:46 |
Last Modified: |
05 Dec 2022 14:39 |
PubMed ID: |
8526531 |
BORIS DOI: |
10.7892/boris.62554 |
URI: |
https://boris.unibe.ch/id/eprint/62554 |
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