Proteomic characterization of the FUS/TLS interactome

Ruepp, Marc-David (24 January 2014). Proteomic characterization of the FUS/TLS interactome (Unpublished). In: Swiss RNA Workshop 2014. Bern, Schweiz. 24.01.2014.

FUS/TLS (fused in sarcoma/translocated in liposarcoma) is a ubiquitously expressed RNA-binding protein, that has been discovered as fused to transcription factors in several human sarcomas and found in protein aggregates in neurons of patients with an inherited form of Amyotrophic Lateral Sclerosis [1].
To date, FUS has been implicated in a variety of cellular processes such as gene expression control, transcriptional regulation, pre-mRNA splicing and miRNA processing [2].
In addition, some evidences link FUS to genome stability control and DNA damage response. In fact, mice lacking FUS are hypersensitive to ionizing radiation and show high levels of chromosome instability and in response to double-strand breaks, FUS gets phosphorylated by the protein kinase ATM [3, 4, 5].
Moreover, upon DNA damage stress, FUS mediates Ebp1 (ErbB3 receptor-binding protein) SUMOylation, a post-translational modification that is required for its onco-suppressive activity, by acting as SUMO E3 ligase [6].
The study aims to investigate the role of FUS in DNA damage response and SUMOylation, two cellular pathways tightly interconnected to each other. Moreover, we will exploit biochemical and mass spectrometry-based approaches in order to identify other potential substrates of the E3 SUMO ligase activity of FUS.
Preliminary results of mass spectrometric identification of FUS interacting proteins, in HEK293 and SHSY5Y cells, highlighted the interaction of FUS with several proteins involved in DNA damage response and many of those have been described already as target of SUMOylation, such as XRCC5, DDX5, PARP1, Nucleophosmin, and others. These evidences strengthen the hypothesis that FUS might represent a link between these pathways, even thou its exact role still needs to be clearly addressed.
[1] Vance C. et al. (2009) Science 323(5918): p. 1208-11
[2] Fiesel FC., Kahle PJ. (2011) FEBS J. 278(19): p. 3550-68
[3] Kuroda M. et al. (2000) Embo J. 19(3): p. 453-62
[4] Hicks GG. et al. (2000) Nat Genet. 24(2):p. 175-9
[5] Gardiner M. et al. (2008) Biochem J. 415(2): p. 297-307
[6] Oh SM. et al. (2010) Oncogene 29(7): p. 1017-30

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