Fytianos, Kleanthis; Rodriguez-Lorenzo, Laura; Clift, Martin J D; Blank, Fabian; Vanhecke, Dimitri; Von Garnier, Christophe; Petri-Fink, Alke; Rothen-Rutishauser, Barbara (2014). Uptake efficiency of surface modified gold nanoparticles does not correlate with functional changes and cytokine secretion in human dendritic cells in vitro. Nanomedicine : nanotechnology, biology, and medicine, 11(3), pp. 633-644. Elsevier 10.1016/j.nano.2014.11.004
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Engineering nanoparticles (NPs) for immune modulation require a thorough understanding of their interaction(s) with cells. Gold NPs (AuNPs) were coated with polyethylene glycol (PEG), polyvinyl alcohol (PVA) or a mixture of both with either positive or negative surface charge to investigate uptake and cell response in monocyte-derived dendritic cells (MDDCs). Inductively coupled plasma optical emission spectrometry and transmission electron microscopy were used to confirm the presence of Au inside MDDCs. Cell viability, (pro-)inflammatory responses, MDDC phenotype, activation markers, antigen uptake and processing were analyzed. Cell death was only observed for PVA-NH2 AuNPs at the highest concentration. MDDCs internalize AuNPs, however, surface modification influenced uptake. Though limited uptake was observed for PEG-COOH AuNPs, a significant tumor necrosis factor-alpha release was induced. In contrast, (PEG+PVA)-NH2 and PVA-NH2 AuNPs were internalized to a higher extent and caused interleukin-1beta secretion. None of the AuNPs caused changes in MDDC phenotype, activation or immunological properties.
Item Type: |
Journal Article (Original Article) |
|---|---|
Division/Institute: |
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > Forschungsbereich Mu50 > Forschungsgruppe Pneumologie (Erwachsene) 04 Faculty of Medicine > Department of Gastro-intestinal, Liver and Lung Disorders (DMLL) > Clinic of Pneumology |
UniBE Contributor: |
Blank, Fabian, von Garnier, Christophe, Rothen-Rutishauser, Barbara |
Subjects: |
600 Technology > 610 Medicine & health |
ISSN: |
1549-9642 |
Publisher: |
Elsevier |
Language: |
English |
Submitter: |
Rahel Holderegger |
Date Deposited: |
10 Mar 2015 09:11 |
Last Modified: |
02 Mar 2023 23:25 |
Publisher DOI: |
10.1016/j.nano.2014.11.004 |
PubMed ID: |
25555350 |
Uncontrolled Keywords: |
Cell viability, Cellular uptake, Dendritic cells, Gold nanoparticles, Immunological properties |
BORIS DOI: |
10.7892/boris.64393 |
URI: |
https://boris.unibe.ch/id/eprint/64393 |
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