DNA hypomethylation and oxidative stress-mediated increase in genomic instability in equine sarcoid-derived fibroblasts

Potocki, Leszek; Lewinska, Anna; Klukowska-Rötzler, Jolanta; Bugno-Poniewierska, Monika; Koch, Christoph; Mählmann, Kathrin; Janda, Jozef; Wnuk, Maciej (2012). DNA hypomethylation and oxidative stress-mediated increase in genomic instability in equine sarcoid-derived fibroblasts. Biochimie, 94(9), pp. 2013-2024. Elsevier Masson SAS 10.1016/j.biochi.2012.05.026

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It is widely accepted that equine sarcoid disease, the most common skin associated neoplasm in equids, is induced by bovine papillomavirus (BPV-1). Although BPV-1 DNA has been found in almost all examined sarcoids so far, its detailed impact on the horse's host cell metabolism is largely unknown. We used equine fibroblast cell lines originating from sarcoid biopsies to study BPV-1-associated changes on DNA methylation status and oxidative stress parameters. Sarcoid-derived fibroblasts manifested increased proliferation in vitro, transcriptional rDNA activity (NORs expression) and DNA hypomethylation compared to control cells. Cells isolated from equine sarcoids suffered from oxidative stress: the expression of antioxidant enzymes was decreased and the superoxide production was increased. Moreover, increased ploidy, oxidative DNA damage and micronuclei formation was monitored in sarcoid cells. We postulate that both altered DNA methylation status and redox milieu may affect genomic stability in BPV-1-infected cells and in turn contribute to sarcoid pathology.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Research Foci > Host-Pathogen Interaction
05 Veterinary Medicine > Department of Clinical Veterinary Medicine (DKV) > Equine Clinic
05 Veterinary Medicine > Department of Clinical Veterinary Medicine (DKV)
05 Veterinary Medicine > Department of Clinical Research and Veterinary Public Health (DCR-VPH) > Experimental Clinical Research
05 Veterinary Medicine > Department of Clinical Research and Veterinary Public Health (DCR-VPH) > Institute of Genetics
05 Veterinary Medicine > Department of Clinical Research and Veterinary Public Health (DCR-VPH)

UniBE Contributor:

Klukowska, Jolanta; Koch, Christoph; Mählmann, Kathrin and Janda, Jozef

Subjects:

500 Science > 590 Animals (Zoology)
600 Technology > 630 Agriculture

ISSN:

0300-9084

Publisher:

Elsevier Masson SAS

Language:

English

Submitter:

Andrea Gassmann-Suter

Date Deposited:

24 Mar 2015 13:57

Last Modified:

17 Aug 2018 15:22

Publisher DOI:

10.1016/j.biochi.2012.05.026

PubMed ID:

22659572

Uncontrolled Keywords:

Horse, Equine sarcoid, Oxidative stress, Genomic instability, DNA methylation

BORIS DOI:

10.7892/boris.65192

URI:

https://boris.unibe.ch/id/eprint/65192

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