An infectious bat-derived chimeric influenza virus harbouring the entry machinery of an influenza A virus.

Juozapaitis, Mindaugas; Aguiar Moreira, Étori; Mena, Ignacio; Giese, Sebastian; Riegger, David; Pohlmann, Anne; Höper, Dirk; Zimmer, Gert; Beer, Martin; García-Sastre, Adolfo; Schwemmle, Martin (2014). An infectious bat-derived chimeric influenza virus harbouring the entry machinery of an influenza A virus. Nature communications, 5, p. 4448. Nature Publishing Group 10.1038/ncomms5448

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In 2012, the complete genomic sequence of a new and potentially harmful influenza A-like virus from bats (H17N10) was identified. However, infectious influenza virus was neither isolated from infected bats nor reconstituted, impeding further characterization of this virus. Here we show the generation of an infectious chimeric virus containing six out of the eight bat virus genes, with the remaining two genes encoding the haemagglutinin and neuraminidase proteins of a prototypic influenza A virus. This engineered virus replicates well in a broad range of mammalian cell cultures, human primary airway epithelial cells and mice, but poorly in avian cells and chicken embryos without further adaptation. Importantly, the bat chimeric virus is unable to reassort with other influenza A viruses. Although our data do not exclude the possibility of zoonotic transmission of bat influenza viruses into the human population, they indicate that multiple barriers exist that makes this an unlikely event.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Research Foci > Host-Pathogen Interaction
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Virology and Immunology
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP)

UniBE Contributor:

Zimmer, Gert

Subjects:

600 Technology > 630 Agriculture

ISSN:

2041-1723

Publisher:

Nature Publishing Group

Language:

English

Submitter:

Barbara Gautschi-Steffen

Date Deposited:

09 Apr 2015 11:31

Last Modified:

06 Mar 2019 13:38

Publisher DOI:

10.1038/ncomms5448

PubMed ID:

25055345

BORIS DOI:

10.7892/boris.66674

URI:

https://boris.unibe.ch/id/eprint/66674

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