Tackling feline infectious peritonitis via reverse genetics

Thiel, Volker Earl; Thiel, Heinz-Jürgen; Tekes, Gergely (2014). Tackling feline infectious peritonitis via reverse genetics. Bioengineered, 5(6), pp. 396-400. Taylor & Francis 10.4161/bioe.32133

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Feline infectious peritonitis (FIP) is caused by feline coronaviruses (FCoVs) and represents one of the most important lethal infectious diseases of cats. To date, there is no efficacious prevention and treatment, and our limited knowledge on FIP pathogenesis is mainly based on analysis of experiments with field isolates. In a recent study, we reported a promising approach to study FIP pathogenesis using reverse genetics. We generated a set of recombinant FCoVs and investigated their pathogenicity in vivo. The set included the type I FCoV strain Black, a type I FCoV strain Black with restored accessory gene 7b, two chimeric type I/type II FCoVs and the highly pathogenic type II FCoV strain 79-1146. All recombinant FCoVs and the reference strain isolates were found to establish productive infections in cats. While none of the type I FCoVs and chimeric FCoVs induced FIP, the recombinant type II FCoV strain 79-1146 was as pathogenic as the parental isolate. Interestingly, an intact ORF 3c was confirmed to be restored in all viruses (re)isolated from FIP-diseased animals.

Item Type:

Journal Article (Original Article)


05 Veterinary Medicine > Research Foci > Host-Pathogen Interaction
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Virology and Immunology
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP)

UniBE Contributor:

Thiel, Volker Earl


600 Technology > 630 Agriculture




Taylor & Francis




Barbara Gautschi-Steffen

Date Deposited:

14 Apr 2015 14:32

Last Modified:

05 Dec 2022 14:45

Publisher DOI:


PubMed ID:


Uncontrolled Keywords:

feline coronavirus, feline infectious peritonitis, reverse genetics, recombinant coronaviruses



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