Koch, Miriam; Clementi, Nina; Rusca, Nicola; Vögele, Paul; Erlacher, Matthias; Polacek, Norbert (2015). The integrity of the G2421-C2395 base pair in the ribosomal E-site is crucial for protein synthesis. RNA biology, 12(1), pp. 70-81. Taylor and Francis 10.1080/15476286.2015.1017218
|
Text
Koch_RNA Biol_2015.pdf - Published Version Available under License Creative Commons: Attribution-Noncommercial (CC-BY-NC). Download (690kB) | Preview |
During the elongation cycle of protein biosynthesis, tRNAs traverse through the ribosome by consecutive binding to the 3 ribosomal binding sites (A-, P-, and E- sites). While the ribosomal A- and P-sites have been functionally well characterized in the past, the contribution of the E-site to protein biosynthesis is still poorly understood in molecular terms. Previous studies suggested an important functional interaction of the terminal residue A76 of E-tRNA with the nucleobase of the universally conserved 23S rRNA residue C2394. Using an atomic mutagenesis approach to introduce non-natural nucleoside analogs into the 23S rRNA, we could show that removal of the nucleobase or the ribose 2'-OH at C2394 had no effect on protein synthesis. On the other hand, our data disclose the importance of the highly conserved E-site base pair G2421-C2395 for effective translation. Ribosomes with a disrupted G2421-C2395 base pair are defective in tRNA binding to the E-site. This results in an impaired translation of genuine mRNAs, while homo-polymeric templates are not affected. Cumulatively our data emphasize the importance of E-site tRNA occupancy and in particular the intactness of the 23S rRNA base pair G2421-C2395 for productive protein biosynthesis.
Item Type: |
Journal Article (Original Article) |
|---|---|
Division/Institute: |
08 Faculty of Science > Department of Chemistry, Biochemistry and Pharmaceutical Sciences (DCBP) |
UniBE Contributor: |
Koch, Miriam, Erlacher, Matthias, Polacek, Norbert |
Subjects: |
500 Science > 570 Life sciences; biology 500 Science > 540 Chemistry |
ISSN: |
1555-8584 |
Publisher: |
Taylor and Francis |
Language: |
English |
Submitter: |
Christina Schüpbach |
Date Deposited: |
01 May 2015 10:56 |
Last Modified: |
05 Dec 2022 14:46 |
Publisher DOI: |
10.1080/15476286.2015.1017218 |
PubMed ID: |
25826414 |
Uncontrolled Keywords: |
23S rRNA; E-site E-tRNA; atomic mutagenesis; ribosome translation |
BORIS DOI: |
10.7892/boris.67761 |
URI: |
https://boris.unibe.ch/id/eprint/67761 |
Actions (login required)
 |
Edit item |