In vivo electroporation mediated gene delivery to the beating heart

Ayuni, Erick L; Gazdhar, Amiq; Giraud, Marie Noelle; Kadner, Alexander; Gugger, Mathias; Cecchini, Marco; Caus, Thierry; Carrel, Thierry P; Schmid, Ralph A; Tevaearai, Hendrik T (2010). In vivo electroporation mediated gene delivery to the beating heart. PLoS ONE, 5(12), e14467. Lawrence, Kans.: Public Library of Science 10.1371/journal.pone.0014467

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Gene therapy may represent a promising alternative strategy for cardiac muscle regeneration. In vivo electroporation, a physical method of gene transfer, has recently evolved as an efficient method for gene transfer. In the current study, we investigated the efficiency and safety of a protocol involving in vivo electroporation for gene transfer to the beating heart. Adult male rats were anesthetised and the heart exposed through a left thoracotomy. Naked plasmid DNA was injected retrograde into the transiently occluded coronary sinus before the electric pulses were applied. Animals were sacrificed at specific time points and gene expression was detected. Results were compared to the group of animals where no electric pulses were applied. No post-procedure arrhythmia was observed. Left ventricular function was temporarily altered only in the group were high pulses were applied; CK-MB (Creatine kinase) and TNT (Troponin T) were also altered only in this group. Histology showed no signs of toxicity. Gene expression was highest at day one. Our results provide evidence that in vivo electroporation with an optimized protocol is a safe and effective tool for nonviral gene delivery to the beating heart. This method may be promising for clinical settings especially for perioperative gene delivery.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Department of Gastro-intestinal, Liver and Lung Disorders (DMLL) > Clinic of Pneumology
04 Faculty of Medicine > Department of Cardiovascular Disorders (DHGE) > Clinic of Cardiovascular Surgery
04 Faculty of Medicine > Service Sector > Institute of Pathology
04 Faculty of Medicine > Department of Dermatology, Urology, Rheumatology, Nephrology, Osteoporosis (DURN) > Clinic of Urology
04 Faculty of Medicine > Department of Gastro-intestinal, Liver and Lung Disorders (DMLL) > Clinic of Thoracic Surgery

UniBE Contributor:

Gazdhar, Amiq Ur Rahman; Kadner, Alexander; Gugger, Mathias; Cecchini, Marco Giovanni; Carrel, Thierry; Schmid, Ralph and Tevaearai, Hendrik

ISSN:

1932-6203

Publisher:

Public Library of Science

Language:

English

Submitter:

Factscience Import

Date Deposited:

04 Oct 2013 14:08

Last Modified:

16 Nov 2015 11:06

Publisher DOI:

10.1371/journal.pone.0014467

PubMed ID:

21209934

Web of Science ID:

000285793600010

BORIS DOI:

10.7892/boris.695

URI:

https://boris.unibe.ch/id/eprint/695 (FactScience: 200411)

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