Invasion and persistence of Mycoplasma bovis in embryonic calf turbinate cells.

Bürki, Sibylle; Gaschen, Véronique; Stoffel, Michael Hubert; Stojiljkovic, Ana; Frey, Joachim; Kühni-Boghenbor, Kathrin; Pilo, Paola (2015). Invasion and persistence of Mycoplasma bovis in embryonic calf turbinate cells. Veterinary research, 46(53), p. 53. Editions scientifiques Elsevier 10.1186/s13567-015-0194-z

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Mycoplasma bovis is a wall-less bacterium causing bovine mycoplasmosis, a disease showing a broad range of clinical manifestations in cattle. It leads to enormous economic losses to the beef and dairy industries. Antibiotic treatments are not efficacious and currently no efficient vaccine is available. Moreover, mechanisms of pathogenicity of this bacterium are not clear, as few virulence attributes are known. Microscopic observations of necropsy material suggest the possibility of an intracellular stage of M. bovis. We used a combination of a gentamicin protection assay, a variety of chemical treatments to block mycoplasmas entry in eukaryotic cells, and fluorescence and transmission electron microscopy to investigate the intracellular life of M. bovis in calf turbinate cells. Our findings indicate that M. bovis invades and persists in primary embryonic calf turbinate cells. Moreover, M. bovis can multiply within these cells. The intracellular phase of M. bovis may represent a protective niche for this pathogen and contribute to its escape from the host's immune defense as well as avoidance of antimicrobial agents.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Research Foci > Host-Pathogen Interaction
05 Veterinary Medicine > Department of Clinical Research and Veterinary Public Health (DCR-VPH) > Veterinary Anatomy
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP)
05 Veterinary Medicine > Department of Clinical Research and Veterinary Public Health (DCR-VPH)
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Veterinary Bacteriology
09 Interdisciplinary Units > Microscopy Imaging Center MIC

Graduate School:

Graduate School for Cellular and Biomedical Sciences (GCB)

UniBE Contributor:

Bürki, Sibylle; Gaschen, Véronique; Stoffel, Michael Hubert; Stojiljkovic, Ana; Frey, Joachim; Kühni, Kathrin and Pilo, Paola

Subjects:

600 Technology > 630 Agriculture
500 Science > 570 Life sciences; biology

ISSN:

0928-4249

Publisher:

Editions scientifiques Elsevier

Language:

English

Submitter:

Paola Pilo

Date Deposited:

20 Apr 2016 08:00

Last Modified:

22 Jan 2019 13:18

Publisher DOI:

10.1186/s13567-015-0194-z

PubMed ID:

25976415

BORIS DOI:

10.7892/boris.69673

URI:

https://boris.unibe.ch/id/eprint/69673

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