Performance of HBsAg point-of-care tests for detection of diagnostic escape-variants in clinical samples

Hirzel, Cédric; Pfister, Stefan; Gorgievski, Meri; Wandeler, Gilles; Zürcher, Samuel (2015). Performance of HBsAg point-of-care tests for detection of diagnostic escape-variants in clinical samples. Journal of clinical virology, 69, pp. 33-35. Elsevier 10.1016/j.jcv.2015.05.024

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BACKGROUND Hepatitis B viruses (HBV) harboring mutations in the a-determinant of the Hepatitis B surface antigen (HBsAg) are associated with reduced reactivity of HBsAg assays. OBJECTIVES To evaluate the sensitivity and specificity of three HBsAg point-of-care tests for the detection of HBsAg of viruses harboring HBsAg mutations. STUDY DESIGN A selection of 50 clinical plasma samples containing HBV with HBsAg mutations was used to evaluate the performance of three HBsAg point-of-care tests (Vikia(®), bioMérieux, Marcy-L'Étoile, France. Alere Determine HBsAg™, Iverness Biomedical Innovations, Köln, Germany. Quick Profile™, LumiQuick Diagnostics, California, USA) and compared to the ARCHITECT HBsAg Qualitative(®) assay (Abbott Laboratories, Sligo, Ireland). RESULTS The sensitivity of the point-of-care tests ranged from 98% to 100%. The only false-negative result occurred using the Quick Profile™ assay with a virus harboring a D144A mutation. CONCLUSIONS The evaluated point-of-care tests revealed an excellent sensitivity in detecting HBV samples harboring HBsAg mutations.

Item Type:

Journal Article (Original Article)


04 Faculty of Medicine > Service Sector > Institute for Infectious Diseases
04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Social and Preventive Medicine
04 Faculty of Medicine > Department of Haematology, Oncology, Infectious Diseases, Laboratory Medicine and Hospital Pharmacy (DOLS) > Clinic of Infectiology

UniBE Contributor:

Hirzel, Cédric; Pfister, Stefan; Gorgievski, Meri; Wandeler, Gilles and Zürcher, Samuel


500 Science > 570 Life sciences; biology
600 Technology > 610 Medicine & health
300 Social sciences, sociology & anthropology > 360 Social problems & social services








Annelies Luginbühl

Date Deposited:

30 Jul 2015 10:00

Last Modified:

01 Aug 2016 02:30

Publisher DOI:


PubMed ID:


Uncontrolled Keywords:

Hepatitis B virus; Diagnostic-escape variants; Hbsag mutations; Point-of-care test; Background




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