Ussher, James E; Phalora, Prabhjeet; Cosgrove, Cormac; Hannaway, Rachel F; Rauch, Andri; Günthard, Huldrych F; Goulder, Philip; Phillips, Rodney E; Willberg, Christian B; Klenerman, Paul (2015). Molecular Analyses Define Vα7.2-Jα33+ MAIT Cell Depletion in HIV Infection: A Case-Control Study. Medicine, 94(29), e1134. Lippincott Williams & Wilkins 10.1097/MD.0000000000001134
|
Text
00005792-201507040-00006.pdf - Published Version Available under License Creative Commons: Attribution (CC-BY). Download (516kB) | Preview |
Mucosal-associated invariant T (MAIT) cells are an abundant antibacterial innate-like lymphocyte population. There are conflicting reports as to their fate in HIV infection. The objective of this study was to determine whether MAIT cells are truly depleted in HIV infection.In this case-control study of HIV-positive patients and healthy controls, quantitative real-time polymerase chain reaction was used to assess the abundance of messenger RNA (mRNA) and genomic DNA (gDNA) encoding the canonical MAIT cell T cell receptor (Vα7.2-Jα33). Comparison was made with flow cytometry.Significant depletion of both Vα7.2-Jα33 mRNA and gDNA was seen in HIV infection. Depletion of Vα7.2+CD161++ T cells was confirmed by flow cytometry. In HIV infection, the abundance of Vα7.2-Jα33 mRNA correlated most strongly with the frequency of Vα7.2+CD161++ cells. No increase was observed in the frequency of Vα7.2+CD161- cells among CD3+CD4- lymphocytes.MAIT cells are depleted from blood in HIV infection as confirmed by independent assays. Significant accumulation of a CD161- MAIT cell population is unlikely. Molecular approaches represent a suitable alternative to flow cytometry-based assays for tracking of MAIT cells in HIV and other settings.
Item Type: |
Journal Article (Original Article) |
|---|---|
Division/Institute: |
04 Faculty of Medicine > Department of Haematology, Oncology, Infectious Diseases, Laboratory Medicine and Hospital Pharmacy (DOLS) > Clinic of Infectiology |
UniBE Contributor: |
Rauch, Andri |
Subjects: |
600 Technology > 610 Medicine & health |
ISSN: |
0025-7974 |
Publisher: |
Lippincott Williams & Wilkins |
Language: |
English |
Submitter: |
Annelies Luginbühl |
Date Deposited: |
30 Jul 2015 10:02 |
Last Modified: |
05 Dec 2022 14:48 |
Publisher DOI: |
10.1097/MD.0000000000001134 |
PubMed ID: |
26200614 |
BORIS DOI: |
10.7892/boris.70550 |
URI: |
https://boris.unibe.ch/id/eprint/70550 |
Actions (login required)
 |
Edit item |