Wöhlke, Anne; Drögemüller, Cord; Kuiper, Heidi; Leeb, Tosso; Distl, Ottmar (2005). Molecular characterization and chromosomal assignment of the bovine glycinamide ribonucleotide formyltransferase (GART) gene on cattle chromosome 1q12.1-q12.2. Gene, 348, pp. 73-81. Elsevier 10.1016/j.gene.2004.12.038
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The mammalian glycinamide ribonucleotide formyltransferase (GART) genes encode a trifunctional polypeptide involved in the de novo purine biosynthesis. We isolated a bacterial artificial chromosome (BAC) clone containing the bovine GART gene and determined the complete DNA sequence of the BAC clone. Cloning and characterization of the bovine GART gene revealed that the bovine gene consists of 23 exons spanning approximately 27 kb. RT-PCR amplification of bovine GART in different organs showed the expression of two GART transcripts in cattle similar to human and mouse. The GART transcripts encode two proteins of 1010 and 433 amino acids, respectively. Eleven single nucleotide polymorphisms (SNPs) were detected in a mutation scan of 24 unrelated animals of three different cattle breeds, including one SNP that affects the amino acid sequence of GART. The chromosomal localization of the gene was determined by fluorescence in situ hybridization. Comparative genome analysis between cattle, human and mouse indicates that the chromosomal location of the bovine GART gene is in agreement with a previously published mapping report.
Item Type: |
Journal Article (Original Article) |
|---|---|
Division/Institute: |
05 Veterinary Medicine > Department of Clinical Research and Veterinary Public Health (DCR-VPH) > Institute of Genetics 05 Veterinary Medicine > Department of Clinical Research and Veterinary Public Health (DCR-VPH) |
UniBE Contributor: |
Drögemüller, Cord, Leeb, Tosso |
Subjects: |
500 Science > 590 Animals (Zoology) 600 Technology > 630 Agriculture 500 Science > 570 Life sciences; biology |
ISSN: |
0378-1119 |
Publisher: |
Elsevier |
Language: |
English |
Submitter: |
Tosso Leeb |
Date Deposited: |
18 Aug 2015 08:34 |
Last Modified: |
05 Dec 2022 14:48 |
Publisher DOI: |
10.1016/j.gene.2004.12.038 |
PubMed ID: |
15777723 |
BORIS DOI: |
10.7892/boris.70803 |
URI: |
https://boris.unibe.ch/id/eprint/70803 |
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