Development of an ultra mini-oxygenator for use in low-volume, buffer-perfused preparations

Berner, Marion; Clément, David; Stadelmann, Mathieu; Kistler, Michael; Boone, Yves; Carrel, Thierry P; Tevaearai, Hendrik T; Longnus, Sarah L (2012). Development of an ultra mini-oxygenator for use in low-volume, buffer-perfused preparations. International journal of artificial organs, 35(4), pp. 308-315. Milano: Wichtig Editore 10.5301/ijao.5000075

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Introduction: Small animal models are widely used in basic research. However, experimental systems requiring extracorporeal circuits are frequently confronted with limitations related to equipment size. This is particularly true for oxygenators in systems with limited volumes. Thus we aimed to develop and validate an ultra mini-oxygenator for low-volume, buffer-perfused systems. Methods: We have manufactured a series of ultra mini-oxygenators with approximately 175 aligned, microporous, polypropylene hollow fibers contained inside a shell, which is sealed at each of the two extremities to isolate perfusate and gas compartments. With this construction, gas passes through hollow fibers, while perfusate circulates around fibers. Performance of ultra mini-oxygenators (oxygen partial pressure (PO2 ), gas and perfusate flow, perfusate pressure and temperature drop) were assessed with modified Krebs-Henseleit buffer in an in vitro perfusion circuit and an ex vivo rat heart preparation. Results: Mean priming volume of ultra mini-oxygenators was 1.2±0.5 mL and, on average, 86±6% of fibers were open (n=17). In vitro, effective oxygenation (PO2=400-500 mmHg) was achieved for all flow rates up to 50 mL/min and remained stable for at least 2 hours (n=5). Oxygenation was also effective and stable (PO2=456±40 mmHg) in the isolated heart preparation for at least 60 minutes ("venous" PO2=151±11 mmHg; n=5). Conclusions: We have established a reproducible procedure for fabrication of ultra mini-oxygenators, which provide reliable and stable oxygenation for at least 60-120 min. These oxygenators are especially attractive for pre-clinical protocols using small, rather than large, animals.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Department of Gastro-intestinal, Liver and Lung Disorders (DMLL) > Clinic of Thoracic Surgery
04 Faculty of Medicine > Department of Cardiovascular Disorders (DHGE) > Clinic of Cardiovascular Surgery
04 Faculty of Medicine > Pre-clinic Human Medicine > Institute for Surgical Technology & Biomechanics ISTB

UniBE Contributor:

Berner, Marion; Clément, David; Stadelmann, Mathieu; Kistler, Michael; Carrel, Thierry; Tevaearai, Hendrik and Longnus, Sarah

Subjects:

600 Technology > 610 Medicine & health
500 Science > 570 Life sciences; biology

ISSN:

0391-3988

Publisher:

Wichtig Editore

Language:

English

Submitter:

Factscience Import

Date Deposited:

04 Oct 2013 14:21

Last Modified:

08 Jul 2016 12:51

Publisher DOI:

10.5301/ijao.5000075

PubMed ID:

22505202

BORIS DOI:

10.7892/boris.7116

URI:

https://boris.unibe.ch/id/eprint/7116 (FactScience: 212290)

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