Visibility of lipid resonances in HR-MAS spectra of brain biopsies subject to spinning rate variation.

Precht, Maria Christina; Diserens, Gaëlle; Oevermann, Anna; Vermathen, Martina; Lang, Johann; Boesch, Christoph Hans; Vermathen, Peter (2015). Visibility of lipid resonances in HR-MAS spectra of brain biopsies subject to spinning rate variation. Biochimica et biophysica acta (BBA) - molecular and cell biology of lipids, 1851(12), pp. 1539-1544. Elsevier 10.1016/j.bbalip.2015.09.003

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Lipid resonances from mobile lipids can be observed by (1)H NMR spectroscopy in multiple tissues and have also been associated with malignancy. In order to use lipid resonances as a marker for disease, a reference standard from a healthy tissue has to be established taking the influence of variable factors like the spinning rate into account. The purpose of our study was to investigate the effect of spinning rate variation on the HR-MAS pattern of lipid resonances in non-neoplastic brain biopsies from different regions and visualize polar and non-polar lipids by fluorescence microscopy using Nile Red staining. (1)H HR-MAS NMR spectroscopy demonstrated higher lipid peak intensities in normal sheep brain pure white matter biopsies compared to mixed white and gray matter biopsies and pure gray matter biopsies. High spinning rates increased the visibility particularly of the methyl resonances at 1.3 and the methylene resonance at 0.89ppm in white matter biopsies stronger compared to thalamus and brainstem biopsies, and gray matter biopsies. The absence of lipid droplets and presence of a large number of myelin sheaths observed in white matter by Nile Red fluorescence microscopy suggest that the observed lipid resonances originate from the macromolecular pool of lipid protons of the myelin sheath's plasma membranes. When using lipid contents as a marker for disease, the variable behavior of lipid resonances in different neuroanatomical regions of the brain and at variable spinning rates should be considered. The findings may open up interesting possibilities for investigating lipids in myelin sheaths.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Research Foci > NeuroCenter
04 Faculty of Medicine > Department of Radiology, Neuroradiology and Nuclear Medicine (DRNN) > Institute of Diagnostic, Interventional and Paediatric Radiology > DCR Magnetic Resonance Spectroscopy and Methodology (AMSM)
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR)
05 Veterinary Medicine > Department of Clinical Veterinary Medicine (DKV)
05 Veterinary Medicine > Department of Clinical Veterinary Medicine (DKV) > DKV - Clinical Radiology
05 Veterinary Medicine > Department of Clinical Veterinary Medicine (DKV) > Small Animal Clinic
05 Veterinary Medicine > Department of Clinical Research and Veterinary Public Health (DCR-VPH) > Experimental Clinical Research
08 Faculty of Science > Departement of Chemistry and Biochemistry
05 Veterinary Medicine > Department of Clinical Research and Veterinary Public Health (DCR-VPH)
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > Forschungsbereich Pavillon 52 > Abt. Magnetresonanz-Spektroskopie und Methodologie, AMSM

Graduate School:

Graduate School for Cellular and Biomedical Sciences (GCB)

UniBE Contributor:

Precht, Maria Christina; Diserens, Gaëlle; Oevermann, Anna; Vermathen, Martina; Lang, Johann; Boesch, Christoph Hans and Vermathen, Peter

Subjects:

500 Science > 540 Chemistry
500 Science > 570 Life sciences; biology
600 Technology > 610 Medicine & health

ISSN:

1388-1981

Publisher:

Elsevier

Language:

English

Submitter:

Maria Christina Precht

Date Deposited:

09 Nov 2015 09:21

Last Modified:

29 May 2017 17:32

Publisher DOI:

10.1016/j.bbalip.2015.09.003

PubMed ID:

26391918

Uncontrolled Keywords:

Gray matter; Lipid; NMR spectroscopy; Nile Red; White matter

BORIS DOI:

10.7892/boris.72774

URI:

https://boris.unibe.ch/id/eprint/72774

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