Planells-Cases, Rosa; Lutter, Darius; Guyader, Charlotte; Gerhards, Nora Merete; Ullrich, Florian; Elger, Deborah A; Kucukosmanoglu, Asli; Xu, Guotai; Voss, Felizia K; Reincke, S Momsen; Stauber, Tobias; Blomen, Vincent A; Vis, Daniel J; Wessels, Lodewyk F; Brummelkamp, Thijn R; Borst, Piet; Rottenberg, Sven; Jentsch, Thomas J (2015). Subunit composition of VRAC channels determines substrate specificity and cellular resistance to Pt-based anti-cancer drugs. The EMBO Journal, 34(24), pp. 2993-3008. EMBO Press 10.15252/embj.201592409
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Although platinum-based drugs are widely used chemotherapeutics for cancer treatment, the determinants of tumor cell responsiveness remain poorly understood. We show that the loss of subunits LRRC8A and LRRC8D of the heteromeric LRRC8 volume-regulated anion channels (VRACs) increased resistance to clinically relevant cisplatin/carboplatin concentrations. Under isotonic conditions, about 50% of cisplatin uptake depended on LRRC8A and LRRC8D, but neither on LRRC8C nor on LRRC8E. Cell swelling strongly enhanced LRRC8-dependent cisplatin uptake, bolstering the notion that cisplatin enters cells through VRAC. LRRC8A disruption also suppressed drug-induced apoptosis independently from drug uptake, possibly by impairing VRAC-dependent apoptotic cell volume decrease. Hence, by mediating cisplatin uptake and facilitating apoptosis, VRAC plays a dual role in the cellular drug response. Incorporation of the LRRC8D subunit into VRAC substantially increased its permeability for cisplatin and the cellular osmolyte taurine, indicating that LRRC8 proteins form the channel pore. Our work suggests that LRRC8D-containing VRACs are crucial for cell volume regulation by an important organic osmolyte and may influence cisplatin/carboplatin responsiveness of tumors.
Item Type: |
Journal Article (Original Article) |
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Division/Institute: |
05 Veterinary Medicine > Research Foci > Host-Pathogen Interaction 05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Animal Pathology 05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) |
Graduate School: |
Graduate School for Cellular and Biomedical Sciences (GCB) |
UniBE Contributor: |
Gerhards, Nora Merete, Rottenberg, Sven |
Subjects: |
500 Science > 570 Life sciences; biology |
ISSN: |
1460-2075 |
Publisher: |
EMBO Press |
Language: |
English |
Submitter: |
Barbara Gautschi-Steffen |
Date Deposited: |
15 Feb 2016 11:35 |
Last Modified: |
05 Dec 2022 14:51 |
Publisher DOI: |
10.15252/embj.201592409 |
PubMed ID: |
26530471 |
Uncontrolled Keywords: |
VSOAC; VSOR; chloride channel; haploid cell screen; swelling‐activated |
BORIS DOI: |
10.7892/boris.75551 |
URI: |
https://boris.unibe.ch/id/eprint/75551 |
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