Methodologies for the Analysis of HCV-Specific CD4(+) T Cells

Lokhande, Megha; Thimme, Robert; Klenerman, Paul; Semmo, Nasser (2015). Methodologies for the Analysis of HCV-Specific CD4(+) T Cells. Frontiers in immunology, 6, p. 57. Frontiers Research Foundation 10.3389/fimmu.2015.00057

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Virus-specific CD4(+) T cells play a major role in viral infections, such as hepatitis C virus (HCV). Viral clearance is associated with vigorous and multi-specific CD4(+) T-cell responses, while chronic infection has been shown to be associated with weak or absent T-cell responses. Most of these studies have used functional assays to analyze virus-specific CD4(+) T-cell responses; however, these and other detection methods have various limitations. Therefore, the important question of whether virus-specific CD4(+) T cells are completely absent or primarily impaired in specific effector functions during chronic infection, has yet to be analyzed in detail. A novel assay, in which virus-specific CD4(+) T-cell frequencies can be determined by de novo CD154 (CD40 ligand) expression in response to viral antigens, can help to overcome some of the limitations of functional assays and restrictions of multimer-based methods. This and other current established methods for the detection of HCV-specific CD4(+) T cells will be discussed in this review.

Item Type:

Journal Article (Review Article)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DBMR Forschung Mu35 > Forschungsgruppe Hepatologie
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DBMR Forschung Mu35 > Forschungsgruppe Hepatologie

04 Faculty of Medicine > Department of Gastro-intestinal, Liver and Lung Disorders (DMLL) > Clinic of Visceral Surgery and Medicine > Hepatology
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR)

UniBE Contributor:

Lokhande, Megha; Klenerman, Paul and Semmo, Nasser

Subjects:

600 Technology > 610 Medicine & health

ISSN:

1664-3224

Publisher:

Frontiers Research Foundation

Language:

English

Submitter:

Lilian Karin Smith-Wirth

Date Deposited:

22 Mar 2016 10:26

Last Modified:

09 Sep 2017 00:33

Publisher DOI:

10.3389/fimmu.2015.00057

PubMed ID:

25767470

Uncontrolled Keywords:

CD154; CD4 T cells; ELISpot; FACS; hepatitis C; tetramers; viral hepatitis

BORIS DOI:

10.7892/boris.77703

URI:

https://boris.unibe.ch/id/eprint/77703

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