The immune response of bovine mammary epithelial cells to live or heat-inactivated Mycoplasma bovis

Zbinden, Christina; Pilo, Paola; Frey, Joachim; Bruckmaier, Rupert; Wellnitz, Olga (2015). The immune response of bovine mammary epithelial cells to live or heat-inactivated Mycoplasma bovis. Veterinary microbiology, 179(3-4), pp. 336-340. Elsevier 10.1016/j.vetmic.2015.07.007

[img] Text
1-s2.0-S0378113515002692-main.pdf - Published Version
Restricted to registered users only
Available under License Publisher holds Copyright.

Download (463kB) | Request a copy
[img]
Preview
Text
Post Print 78517.pdf - Accepted Version
Available under License Creative Commons: Attribution-Noncommercial-No Derivative Works (CC-BY-NC-ND).

Download (244kB) | Preview

Mycoplasma bovis is an emerging bacterial agent causing bovine mastitis. Although these cell wall-free bacteria lack classical virulence factors, they are able to activate the immune system of the host. However, effects on the bovine mammary immune system are not yet well characterized and detailed knowledge would improve the prevention and therapy of mycoplasmal mastitis. The aim of this study was to investigate the immunogenic effects of M. bovis on the mammary gland in an established primary bovine mammary epithelial cell (bMEC) culture system. Primary bMEC of four different cows were challenged with live and heat-inactivated M. bovis strain JF4278 isolated from acute bovine mastitis, as well as with the type strain PG45. The immune response was evaluated 6 and 24h after mycoplasmal challenge by measuring the relative mRNA expression of selected immune factors by quantitative PCR. M. bovis triggered an immune response in bMEC, reflected by the upregulation of tumor necrosis factor-α, interleukin(IL)-1β, IL-6, IL-8, lactoferrin, Toll-like receptor-2, RANTES, and serum amyloid A mRNA. Interestingly, this cellular reaction was only observed in response to live, but not to heat-inactivated M. bovis, in contrast to other bacterial pathogens of mastitis such as Staphylococcus aureus. This study provides evidence that bMEC exhibit a strong inflammatory reaction in response to live M. bovis. The lack of a cellular response to heat-inactivated M. bovis supports the current hypothesis that mycoplasmas activate the immune system through secreted secondary metabolites.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Research Foci > Host-Pathogen Interaction
05 Veterinary Medicine > Department of Clinical Research and Veterinary Public Health (DCR-VPH) > Veterinary Physiology
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP)
05 Veterinary Medicine > Department of Clinical Research and Veterinary Public Health (DCR-VPH)
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Veterinary Bacteriology

Graduate School:

Graduate School for Cellular and Biomedical Sciences (GCB)

UniBE Contributor:

Zbinden, Christina; Pilo, Paola; Frey, Joachim; Bruckmaier, Rupert and Wellnitz, Olga

Subjects:

600 Technology > 630 Agriculture

ISSN:

0378-1135

Publisher:

Elsevier

Language:

English

Submitter:

Barbara Gautschi-Steffen

Date Deposited:

01 Mar 2016 10:07

Last Modified:

10 Sep 2017 01:40

Publisher DOI:

10.1016/j.vetmic.2015.07.007

PubMed ID:

26211967

Uncontrolled Keywords:

Bovine mammary epithelial cells; Innate immune response; Mastitis; Mycoplasma bovis

BORIS DOI:

10.7892/boris.78517

URI:

https://boris.unibe.ch/id/eprint/78517

Actions (login required)

Edit item Edit item
Provide Feedback