Force-controlled patch clamp of beating cardiac cells.

Ossola, Dario; Amarouch, Mohamed Yassine; Behr, Pascal; Vörös, János; Abriel, Hugues; Zambelli, Tomaso (2015). Force-controlled patch clamp of beating cardiac cells. Nano letters, 15(3), pp. 1743-1750. American Chemical Society 10.1021/nl504438z

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From its invention in the 1970s, the patch clamp technique is the gold standard in electrophysiology research and drug screening because it is the only tool enabling accurate investigation of voltage-gated ion channels, which are responsible for action potentials. Because of its key role in drug screening, innovation efforts are being made to reduce its complexity toward more automated systems. While some of these new approaches are being adopted in pharmaceutical companies, conventional patch-clamp remains unmatched in fundamental research due to its versatility. Here, we merged the patch clamp and atomic force microscope (AFM) techniques, thus equipping the patch-clamp with the sensitive AFM force control. This was possible using the FluidFM, a force-controlled nanopipette based on microchanneled AFM cantilevers. First, the compatibility of the system with patch-clamp electronics and its ability to record the activity of voltage-gated ion channels in whole-cell configuration was demonstrated with sodium (NaV1.5) channels. Second, we showed the feasibility of simultaneous recording of membrane current and force development during contraction of isolated cardiomyocytes. Force feedback allowed for a gentle and stable contact between AFM tip and cell membrane enabling serial patch clamping and injection without apparent cell damage.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DBMR Forschung Mu35 > Forschungsgruppe Ionenkanalkrankheiten
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DBMR Forschung Mu35 > Forschungsgruppe Ionenkanalkrankheiten

UniBE Contributor:

Amarouch, Mohamed Yassine and Abriel, Hugues

Subjects:

600 Technology > 610 Medicine & health

ISSN:

1530-6984

Publisher:

American Chemical Society

Language:

English

Submitter:

Verena de Serra Frazao-Bill

Date Deposited:

13 Apr 2016 07:45

Last Modified:

13 Apr 2016 07:45

Publisher DOI:

10.1021/nl504438z

PubMed ID:

25639960

Uncontrolled Keywords:

FluidFM; NaV1.5 channels; Whole-cell patch clamp; atomic force microscope; cardiomyocytes; microchanneled AFM cantilevers

BORIS DOI:

10.7892/boris.80624

URI:

https://boris.unibe.ch/id/eprint/80624

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