Structure determination of channel and transport proteins by high-resolution microscopy techniques

Meury, Marcel; Harder, Daniel; Ucurum, Zöhre; Boggavarapu, Rajendra; Jeckelmann, Jean-Marc; Fotiadis, Dimitrios (2011). Structure determination of channel and transport proteins by high-resolution microscopy techniques. Biological chemistry, 392(1-2), pp. 143-50. Berlin: Walter de Gruyter 10.1515/BC.2011.004

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High-resolution microscopy techniques provide a plethora of information on biological structures from the cellular level down to the molecular level. In this review, we present the unique capabilities of transmission electron and atomic force microscopy to assess the structure, oligomeric state, function and dynamics of channel and transport proteins in their native environment, the lipid bilayer. Most importantly, membrane proteins can be visualized in the frozen-hydrated state and in buffer solution by cryo-transmission electron and atomic force microscopy, respectively. We also illustrate the potential of the scintillation proximity assay to study substrate binding of detergent-solubilized transporters prior to crystallization and structural characterization.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Biochemistry and Molecular Medicine

UniBE Contributor:

Meury, Marcel; Harder, Daniel; Ucurum Fotiadis, Zöhre; Boggavarapu, Venkata Ravi Naga Rajendr; Jeckelmann, Jean-Marc and Fotiadis, Dimitrios José

ISSN:

1431-6730

Publisher:

Walter de Gruyter

Language:

English

Submitter:

Factscience Import

Date Deposited:

04 Oct 2013 14:23

Last Modified:

27 Apr 2018 09:41

Publisher DOI:

10.1515/BC.2011.004

PubMed ID:

21194358

Web of Science ID:

000286183700016

BORIS DOI:

10.7892/boris.8146

URI:

https://boris.unibe.ch/id/eprint/8146 (FactScience: 213633)

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