Evidence for two protein coding transcripts at the Igf2as locus

Duart Garcia, Carolina; Plattet, Philippe; Bruggmann, Rémy; Simillion, Cedric André Marie; Irene, Keller; Göran, Andersson; Braunschweig, Martin (2016). Evidence for two protein coding transcripts at the Igf2as locus. Gene Reports, 4, pp. 60-66. Elsevier 10.1016/j.genrep.2016.04.003

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The insulin-like growth factor 2 antisense (Igf2as) gene is part of the Ins-Igf2-H19 imprinted gene cluster. The function of the paternally expressed Igf2as is still elusive. In our previous work, we showed that Igf2as transcripts were located in the cytoplasm of C2C12 mouse myoblast cells, associated with polysomes and polyadenylated suggesting that Igf2as is protein coding. In the present work, the protein coding capacity of Igf2as was investigated. We demonstrate for the first time the existence of a polypeptide translated from an Igf2as construct. Furthermore, an RNA-Seq analysis was performed using RNA prepared from skeletal muscles of newborn wild-type and ∆ DMR1-U2 mice to further elucidate the function of Igf2as transcripts. We found no evidence for a regulatory role of Igf2as in the imprinted gene cluster. Interestingly, the RNA-Seq analysis indicated that Igf2as plays a role in the energy metabolism, the cell cycle, histone acetylation and muscle contraction pathways. Our Igf2as investigations further elucidated that there are two distinct Igf2as transcripts corresponding to two putative ORFs.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR)
05 Veterinary Medicine > Department of Clinical Research and Veterinary Public Health (DCR-VPH) > Experimental Clinical Research
05 Veterinary Medicine > Department of Clinical Research and Veterinary Public Health (DCR-VPH) > Institute of Genetics
05 Veterinary Medicine > Department of Clinical Research and Veterinary Public Health (DCR-VPH)

UniBE Contributor:

Duart Garcia, Carolina; Plattet, Philippe; Bruggmann, Rémy; Simillion, Cedric André Marie and Braunschweig, Martin

Subjects:

500 Science > 590 Animals (Zoology)
600 Technology > 630 Agriculture
600 Technology > 610 Medicine & health
500 Science > 570 Life sciences; biology

ISSN:

2452-0144

Publisher:

Elsevier

Language:

English

Submitter:

Tosso Leeb

Date Deposited:

08 Jul 2016 11:49

Last Modified:

08 Sep 2017 14:04

Publisher DOI:

10.1016/j.genrep.2016.04.003

BORIS DOI:

10.7892/boris.82017

URI:

https://boris.unibe.ch/id/eprint/82017

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