Luidalepp, Hannes; Berger, Stefan; Joss, Oliver; Tenson, Tanel; Polacek, Norbert (2016). Ribosome Shut-Down by 16S rRNA Fragmentation in Stationary-Phase Escherichia coli. Journal of molecular biology, 428(10), pp. 2237-2247. Elsevier 10.1016/j.jmb.2016.01.033
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Stationary-phase bacterial cells are characterized by vastly reduced metabolic activities yielding a dormant-like phenotype. Several hibernation programs ensure the establishment and maintenance of this resting growth state. Some of the stationary phase-specific modulations affect the ribosome and its translational activity directly. In stationary-phase Escherichia coli, we observed the appearance of a 16S rRNA fragmentation event at the tip of helix 6 within the small ribosomal subunit (30S). Stationary-phase 30S subunits showed markedly reduced activities in protein biosynthesis. On the other hand, the functional performance of stationary-phase large ribosomal subunits (50S) was indistinguishable from particles isolated from exponentially growing cells. Introduction of the 16S rRNA cut in vitro at helix 6 of exponential phase 30S
subunits renders them less efficient in protein biosynthesis. This indicates that the helix 6 fragmentation is necessary and sufficient to attenuate translational activities of 30S ribosomal subunits. These results suggest that stationary phase-specific cleavage of 16S rRNA within the 30S subunit is an efficient means to reduce global translation activities under non-proliferating growth conditions.
Item Type: |
Journal Article (Original Article) |
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Division/Institute: |
08 Faculty of Science > Department of Chemistry, Biochemistry and Pharmaceutical Sciences (DCBP) |
UniBE Contributor: |
Luidalepp, Hannes, Berger, Stefan, Joss, Oliver, Polacek, Norbert |
Subjects: |
500 Science > 570 Life sciences; biology 500 Science > 540 Chemistry |
ISSN: |
0022-2836 |
Publisher: |
Elsevier |
Language: |
English |
Submitter: |
Christina Schüpbach |
Date Deposited: |
08 Jul 2016 12:14 |
Last Modified: |
05 Dec 2022 14:56 |
Publisher DOI: |
10.1016/j.jmb.2016.01.033 |
BORIS DOI: |
10.7892/boris.82146 |
URI: |
https://boris.unibe.ch/id/eprint/82146 |
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