Ribosome Shut-Down by 16S rRNA Fragmentation in Stationary-Phase Escherichia coli

Luidalepp, Hannes; Berger, Stefan; Joss, Oliver; Tenson, Tanel; Polacek, Norbert (2016). Ribosome Shut-Down by 16S rRNA Fragmentation in Stationary-Phase Escherichia coli. Journal of molecular biology, 428(10), pp. 2237-2247. Elsevier 10.1016/j.jmb.2016.01.033

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Stationary-phase bacterial cells are characterized by vastly reduced metabolic activities yielding a dormant-like phenotype. Several hibernation programs ensure the establishment and maintenance of this resting growth state. Some of the stationary phase-specific modulations affect the ribosome and its translational activity directly. In stationary-phase Escherichia coli, we observed the appearance of a 16S rRNA fragmentation event at the tip of helix 6 within the small ribosomal subunit (30S). Stationary-phase 30S subunits showed markedly reduced activities in protein biosynthesis. On the other hand, the functional performance of stationary-phase large ribosomal subunits (50S) was indistinguishable from particles isolated from exponentially growing cells. Introduction of the 16S rRNA cut in vitro at helix 6 of exponential phase 30S subunits renders them less efficient in protein biosynthesis. This indicates that the helix 6 fragmentation is necessary and sufficient to attenuate translational activities of 30S ribosomal subunits. These results suggest that stationary phase-specific cleavage of 16S rRNA within the 30S subunit is an efficient means to reduce global translation activities under non-proliferating growth conditions.

Item Type:

Journal Article (Original Article)

Division/Institute:

08 Faculty of Science > Departement of Chemistry and Biochemistry

UniBE Contributor:

Luidalepp, Hannes; Berger, Stefan; Joss, Oliver and Polacek, Norbert

Subjects:

500 Science > 570 Life sciences; biology
500 Science > 540 Chemistry

ISSN:

0022-2836

Publisher:

Elsevier

Language:

English

Submitter:

Christina Schüpbach

Date Deposited:

08 Jul 2016 12:14

Last Modified:

08 Jul 2016 12:14

Publisher DOI:

10.1016/j.jmb.2016.01.033

BORIS DOI:

10.7892/boris.82146

URI:

https://boris.unibe.ch/id/eprint/82146

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