Effects and uptake of gold nanoparticles deposited at the air–liquid interface of a human epithelial airway model

Brandenberger, Christina; Rothen-Rutishauser, Barbara; Mühlfeld, Christian; Schmid, Otmar; Ferron, George A; Maier, Konrad Ludwig; Gehr, Peter; Lenz, Anke Gabriele (2010). Effects and uptake of gold nanoparticles deposited at the air–liquid interface of a human epithelial airway model. Toxicology and Applied Pharmacology, 242(1), pp. 56-65. Amsterdam: Elsevier 10.1016/j.taap.2009.09.014

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The impact of nanoparticles (NPs) in medicine and biology has increased rapidly in recent years. Gold NPs have advantageous properties such as chemical stability, high electron density and affinity to biomolecules, making them very promising candidates as drug carriers and diagnostic tools. However, diverse studies on the toxicity of gold NPs have reported contradictory results. To address this issue, a triple cell co-culture model simulating the alveolar lung epithelium was used and exposed at the air-liquid interface. The cell cultures were exposed to characterized aerosols with 15 nm gold particles (61 ng Au/cm2 and 561 ng Au/cm2 deposition) and incubated for 4 h and 24 h. Experiments were repeated six times. The mRNA induction of pro-inflammatory (TNFalpha, IL-8, iNOS) and oxidative stress markers (HO-1, SOD2) was measured, as well as protein induction of pro- and anti-inflammatory cytokines (IL-1, IL-2, IL-4, IL-6, IL-8, IL-10, GM-CSF, TNFalpha, INFgamma). A pre-stimulation with lipopolysaccharide (LPS) was performed to further study the effects of particles under inflammatory conditions. Particle deposition and particle uptake by cells were analyzed by transmission electron microscopy and design-based stereology. A homogeneous deposition was revealed, and particles were found to enter all cell types. No mRNA induction due to particles was observed for all markers. The cell culture system was sensitive to LPS but gold particles did not cause any synergistic or suppressive effects. With this experimental setup, reflecting the physiological conditions more precisely, no adverse effects from gold NPs were observed. However, chronic studies under in vivo conditions are needed to entirely exclude adverse effects.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Anatomy
04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Anatomy > Topographical and Clinical Anatomy

UniBE Contributor:

Brandenberger, Christina; Rothen-Rutishauser, Barbara; Mühlfeld, Christian and Gehr, Peter

Subjects:

600 Technology > 610 Medicine & health

ISSN:

0041-008X

Publisher:

Elsevier

Language:

English

Submitter:

Factscience Import

Date Deposited:

04 Oct 2013 14:07

Last Modified:

24 Nov 2016 17:14

Publisher DOI:

10.1016/j.taap.2009.09.014

PubMed ID:

19796648

Web of Science ID:

000272434800007

URI:

https://boris.unibe.ch/id/eprint/85 (FactScience: 195480)

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