High resolution microscopy reveals an unusual architecture of the Plasmodium berghei endoplasmic reticulum.

Kaiser, Gesine; De Niz Hidalgo, Mariana Isabel; Zuber, Benoît; Burda, Paul-Christian; Kornmann, Benoît; Limenitakis Stanway, Rebecca; Heussler, Volker (2016). High resolution microscopy reveals an unusual architecture of the Plasmodium berghei endoplasmic reticulum. Molecular microbiology, 102(5), pp. 775-791. Blackwell Science 10.1111/mmi.13490

[img]
Preview
Text
Kaiser_et_al-2016-Molecular_Microbiology.pdf - Published Version
Available under License Creative Commons: Attribution-Noncommercial (CC-BY-NC).

Download (6MB) | Preview

To fuel the tremendously fast replication of Plasmodium liver stage parasites, the endoplasmic reticulum (ER) must play a critical role as a major site of protein and lipid biosynthesis. In this study, we analysed the parasite's ER morphology and function. Previous studies exploring the parasite ER have mainly focused on the blood stage. Visualizing the Plasmodium berghei ER during liver stage development, we found that the ER forms an interconnected network throughout the parasite with perinuclear and peripheral localizations. Surprisingly, we observed that the ER additionally generates huge accumulations. Using stimulated emission depletion microscopy and serial block-face scanning electron microscopy, we defined ER accumulations as intricate dense networks of ER tubules. We provide evidence that these accumulations are functional subdivisions of the parasite ER, presumably generated in response to elevated demands of the parasite, potentially consistent with ER stress. Compared to higher eukaryotes, Plasmodium parasites have a fundamentally reduced unfolded protein response machinery for reacting to ER stress. Accordingly, parasite development is greatly impaired when ER stress is applied. As parasites appear to be more sensitive to ER stress than are host cells, induction of ER stress could potentially be used for interference with parasite development.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Anatomy
08 Faculty of Science > Department of Biology > Institute of Cell Biology > Malaria
08 Faculty of Science > Department of Biology > Institute of Cell Biology
09 Interdisciplinary Units > Microscopy Imaging Center (MIC)

Graduate School:

Graduate School for Cellular and Biomedical Sciences (GCB)

UniBE Contributor:

Kaiser, Gesine; De Niz Hidalgo, Mariana Isabel; Zuber, Benoît; Burda, Paul-Christian; Limenitakis Stanway, Rebecca and Heussler, Volker

Subjects:

600 Technology > 610 Medicine & health
500 Science > 570 Life sciences; biology

ISSN:

0950-382X

Publisher:

Blackwell Science

Funders:

[42] Schweizerischer Nationalfonds
[UNSPECIFIED] SystemsX grant 51RTPO_151032
[81] Evimalar

Language:

English

Submitter:

Volker Heussler

Date Deposited:

07 Oct 2016 12:52

Last Modified:

14 Feb 2019 18:03

Publisher DOI:

10.1111/mmi.13490

PubMed ID:

27566438

BORIS DOI:

10.7892/boris.89165

URI:

https://boris.unibe.ch/id/eprint/89165

Actions (login required)

Edit item Edit item
Provide Feedback