Reisenhofer, Miriam Helen; Pannicke, Thomas; Reichenbach, Andreas; Enzmann, Volker (2016). Characteristics of Müller glial cells in MNU-induced retinal degeneration. (In Press). Visual neuroscience, 33 Cambridge University Press 10.1017/S0952523816000109
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Retinal Müller glial cells have been shown to undergo reactive gliosis in a variety of retinal diseases. Upregulation of glial fibrillary acidic protein (GFAP) is a hallmark of Müller cell activation. Reactive gliosis after retinal detachment or ischemia/reperfusion is characterized by hypertrophy and downregulation of inwardly rectifying K+ (Kir) currents. However, this kind of physiological alteration could not be detected in slowly progressing retinal degenerations. The photoreceptor toxin N-methyl-N-nitrosourea (MNU) leads to the rapid loss of cells in the outer nuclear layer and subsequent Müller cell activation. Here, we investigated whether Müller cells from MNU-treated mice exhibit reactive gliosis. We found that Müller cells showed increased GFAP expression and increased membrane capacitance, indicating hypertrophy. Membrane potential and Kir channel-mediated K+ currents were not significantly altered whereas Kir4.1 mRNA expression and Kir-mediated inward current densities were markedly decreased. This suggests that MNU-induced Müller cell gliosis is characterized by plasma membrane increase without alteration in the membrane content of Kir channels. Taken together, our findings show that Müller cells of MNU-treated mice are reactive and respond with a form of gliosis which is characterized by cellular hypertrophy but no changes in Kir current amplitudes.
Item Type: |
Journal Article (Original Article) |
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Division/Institute: |
04 Faculty of Medicine > Department of Head Organs and Neurology (DKNS) > Clinic of Ophthalmology |
Graduate School: |
Graduate School for Cellular and Biomedical Sciences (GCB) |
UniBE Contributor: |
Reisenhofer, Miriam Helen, Enzmann, Volker |
Subjects: |
500 Science > 570 Life sciences; biology 600 Technology > 610 Medicine & health |
ISSN: |
0952-5238 |
Publisher: |
Cambridge University Press |
Language: |
English |
Submitter: |
Volker Enzmann |
Date Deposited: |
18 Nov 2016 12:59 |
Last Modified: |
05 Dec 2022 14:59 |
Publisher DOI: |
10.1017/S0952523816000109 |
BORIS DOI: |
10.7892/boris.89552 |
URI: |
https://boris.unibe.ch/id/eprint/89552 |
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