ABC transporters and xenobiotic defense systems in early life stages of rainbow trout (Oncorhynchus mykiss)

Kropf, Christian; Segner, Helmut; Fent, Karl (2016). ABC transporters and xenobiotic defense systems in early life stages of rainbow trout (Oncorhynchus mykiss). Comparative biochemistry and physiology. Part C - toxicology and pharmacology, 185-186, pp. 45-56. Elsevier 10.1016/j.cbpc.2016.02.006

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Embryos of oviparous fish, in contrast to (ovo) viviparous species, develop in the aquatic environment, and therefore need solute transport systems at their body surfaces for maintaining internal homeostasis and defending against potentially harmful substances. We hypothesized that solute transporters undergo changes in tissue distribution from the embryo to the larval stage. We therefore studied the mRNA profiles of eight ABC transporters (abcb1a, abcb1b, abcc1, abcc2, abcc3, abcc4, abcc5, abcg2) and three solute carriers (oatp1d, putative oatp2 putative, mate1) in different body regions (head, yolk sac epithelium, abdominal viscera, skin/muscles) of developing rainbow trout. Additionally, we investigated mRNA levels of phase I (cyp1a, cyp3a) and phase II (gstp, putative ugt1, putative ugt2) biotransformation enzymes. The study covered the developmental period from the eleuthero-embryo stage to the first-feeding larval stage (1-20days post-hatch, dph). At 1dph, transcripts of abcc2, abcc4, abcg2, cyp3a, gstp, putative mate1, and putative oatp2 occurred primarily in the yolk sac epithelium, whereas at later stages expression of these genes was predominantly observed in the abdominal viscera. The functional activity of ABC transporters in fish early life stages was assessed by rhodamine B accumulation assays. Finally, we investigated the potential impact of xenobiotics (clotrimazole, clofibric acid) on the ABC and biotransformation systems of trout early life stages. While clofibric acid had no effect, clotrimazole lead to an increased rhodamine B accumulation. The results provide evidence that the transition from the eleuthero-embryo to the larval stage is accompanied by a major alteration in tissue expression of ABC transporters.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP)
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Center for Fish and Wildlife Health (FIWI)

Graduate School:

Graduate School for Cellular and Biomedical Sciences (GCB)

UniBE Contributor:

Kropf, Christian and Segner, Helmut

Subjects:

600 Technology > 630 Agriculture
500 Science > 570 Life sciences; biology

ISSN:

1532-0456

Publisher:

Elsevier

Funders:

[42] Schweizerischer Nationalfonds

Language:

English

Submitter:

Christian Kropf

Date Deposited:

13 Feb 2017 16:43

Last Modified:

13 Feb 2017 16:43

Publisher DOI:

10.1016/j.cbpc.2016.02.006

PubMed ID:

26945521

Uncontrolled Keywords:

ABC transporter; Biotransformation; Early life stage; Oncorhynchus mykiss; Solute carriers; Yolk sac

BORIS DOI:

10.7892/boris.91335

URI:

https://boris.unibe.ch/id/eprint/91335

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